相关实验视频

Updated: May 13, 2025

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

19.8K

转录相关突变性驱动tRNA微进化有多大?

Hector Banos, Ling Wang, Corinne Simonti

    bioRxiv : the preprint server for biology
    |April 16, 2025
    PubMed
    概括

    转录相关突变发生 (TAM) 驱动转移RNA (tRNA) 的遗传变异. 我们的研究表明,TAM显著塑造了人群中的tRNA等位基因变异,挑战了以前的模型.

    更多相关视频

    Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms09:30

    Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

    Published on: September 13, 2018

    9.4K
    An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity07:46

    An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

    Published on: October 8, 2018

    6.9K

    相关概念视频

    Viral Mutations00:36

    Viral Mutations

    A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
    32.0K
    Bacterial Transcription01:53

    Bacterial Transcription

    RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
    Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
    27.7K
    Mutations01:39

    Mutations

    Overview
    77.4K
    Genome Copying Errors02:46

    Genome Copying Errors

    DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
    4.1K
    Gene Evolution - Fast or Slow?02:05

    Gene Evolution - Fast or Slow?

    The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
    In contrast, regions which code...
    7.0K
    tRNA Activation02:26

    tRNA Activation

    Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
    18.7K