在急性和长期持久感染期间,DNA病毒群体的流放动态
bioRxiv : the preprint server for biology
|April 16, 2025
概括
鼠类多重瘤病毒 (muPyV) 在小鼠中产生长期感染,常有低水平的脱落和罕见的高水平的重新激活事件. 这些发现解释了多病毒的持久性和传播潜力.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 了解宿主病毒感染动态对于解释病毒持久性和传播至关重要.
- 聚瘤病毒感染可能是持久的,并导致疾病,特别是在免疫受损的个体.
- 由于持续感染,多瘤病毒的建立,维持和重新激活的机制仍然不太清楚.
研究的目的:
- 通过使用条形码病毒系统,研究小鼠多瘤病毒 (muPyV) 感染的宿主内部动态.
- 描述muPyV.的长期脱落模式和特定器官储存库.
- 阐明多病毒持久性和传播潜力的基础机制.
主要方法:
- 开发一种使用条形码muPyV.使用的非侵入性病毒泄漏试验.
- 应用大规模并行测序技术来追踪病毒基因组.
- 利用新的计算方法来分析小鼠的长期感染数据.
主要成果:
- 数以千计的带有条形码的muPyV在单个小鼠中感染并长期存在.
- 在整个感染过程中,观察到大量病毒条形码的持续的低水平泄露.
- 发生了一些条形码的插曲性,高水平的泄露,这导致随着时间的推移减少了多样性.
- 确定了具有独特条形码丰度模式的器官特异性病毒储存库.
结论:
- 多种病毒平衡连续传播潜力与储库驱动的重新激活.
- 观察到的脱落模式表明持续的低水平感染,偶尔会出现复制爆发.
- 这些机制为多病毒的无处不在和长期持久性提供了基础,这在DNA病毒中很常见.
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