重复性肠球菌病的临床和基因组特征:多中心前性队列研究 (VENOUS)
bioRxiv : the preprint server for biology
|April 16, 2025
概括
反复性肠球菌血流感染与不良结果有关. 基因组分析揭示了E. faecium*和E. faecalis*的物种特异性适应,突出了对持久性感染的潜在遗传倾向.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 复发性肠球菌血流感染对患者的不良结果构成重大风险.
- 用于调查这些具有挑战性的感染,使用了抗万科胺素肠球菌细菌病结局研究 (VENOUS) 队列 (2016-2022年).
研究的目的:
- 进行复发性肠球菌血流感染患者的临床和基因组表征.
- 为了确定潜在的基因组因素,有助于持续或复发的肠球菌病.
主要方法:
- 定义"持久"的细菌病是指尽管积极治疗,但在第四天缺乏清除,而"复发"是指清除后再培养.
- 采用全基因组测序 (短读和长读) 和混合组件进行隔离分析.
- 匹配了反抗性病例 (n=46) 与非反抗性对照 (2:1比率).
主要成果:
- 从41名患者中确定了46例反抗性感染.
- 患有持续性细菌病的患者更频繁地被录入ICU.
- 长期感染的E. faecalis*菌株显示碳水化合物利用基因增加.
- 在*E. faecium*和*E. faecalis*之间的突变基因中,不同功能组的表现表明了特定物种的适应.
结论:
- 反抗性肠球菌分离物的基因组多样性表明,菌株特定的特征不是持久性的主要驱动因素.
- *E. faecium*可能具有引起持久性感染的遗传预适应.
- 特定部位的变异表明差异性基因表达在细菌病期间的肠球菌适应中起作用.
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