相关实验视频

Updated: Jun 10, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

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核蛋白标和基因组DNA的对分析通过单细胞西区块和单细胞PCR

Ana E Gomez Martinez, Trinh Lam, Amy E Herr

    bioRxiv : the preprint server for biology
    |April 16, 2025
    PubMed
    概括

    我们开发了SplitBlot,这是一种用于同时进行单细胞基因组DNA和蛋白质分析的新型微流体检测. 这个工具将基于PCR的DNA放大与芯片上的西部涂抹结合起来,以获得基因型-表型相关性.

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    Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease09:20

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    Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution10:03

    Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

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    DNA Isolation01:24

    DNA Isolation

    DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
    Southern Blot02:57

    Southern Blot

    Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
    Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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    PCR - Polymerase Chain Reaction

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