蛋白相互作用网络分析揭示了 Prp19 剪接因子在包含内子和缺乏内子的基因的转录中的作用
bioRxiv : the preprint server for biology
|April 16, 2025
概括
这项研究揭示了拼接因子Prp19在启动基因转录中起着新的作用. 削弱Prp19通过影响促进体结合,减少了包含内子和缺乏内子的基因的转录.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 酵母遗传学 酵母遗传学
背景情况:
- 基因循环对于转录至关重要,它涉及基因末端和转录因子之间的相互作用.
- 之前的工作确定了TFIIB通过终结复合体促进基因循环.
- 蛋白质-蛋白质相互作用在稳定基因循环中的作用,特别是关于拼接因子的作用,仍然不清楚.
研究的目的:
- 为了确定稳定基因循环的蛋白质相互作用.
- 调查终结因子与拼接机械相互作用的作用.
- 阐明 Prp19 在转录周期中的功能.
主要方法:
- 从染色质中结尾复合物的亲和力净化和质谱学.
- 定量蛋白质组分析以确定相互作用的蛋白质.
- 辅酶介导的Prp19和染色体免疫沉 (ChIP) 试验的耗尽.
主要成果:
- 终止因子与TFIID,SAGA和拼接因子 (Prp19,Prp43,Sub2,Snu114,Brr2,Smb1) 相互作用. 终止因子与这些因子相互作用.
- Prp19与TFIID,CPF和RSC复合体相互作用,仅在染色体中.
- Prp19的枯竭减少了转录和TBP促进体的招募;ChIP显示Prp19在基因促进体和下游区域.
结论:
- Prp19是转录启动和终止复合物的新型相互作用者.
- Prp19的作用比以前想象的要广泛,它会影响包含内子和缺乏内子的基因.
- Prp19与酵母中RNAPII转录的启动步骤有关.
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