Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Orthogonal nanopores cross-validation for multiplex single-molecule profiling.

Chemical science·2026
Same author

A Dynamic Contour Evolution Algorithm for Cell Segmentation and Synaptic Tracking under Occlusion.

Chemical & biomedical imaging·2026
Same author

Photoresponsive DNA steganography for secure information transmission by nanopore.

National science review·2026
Same author

Nanopore Fingerprinting of Neurodegenerative Proteins and Phosphoproteins within a Minute.

JACS Au·2026
Same author

Wireless nanopore electrodes (WNEs): from a non-contact conductive tip to applications in electroanalysis and electrocatalysis.

Chemical science·2026
Same author

Nanopipette Electrochemistry.

Chemical reviews·2025

相关实验视频

Updated: Jun 14, 2026

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.5K

探索一种固态纳米孔方法,用于从单细胞中检测单分子蛋白质.

Zi-Qi Zhou1, Shao-Chuang Liu1, Jia Wang1

  • 1Molecular Sensing and Imaging Center, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China yitaolong@nju.edu.cn shaochuangliu@nju.edu.cn.

Chemical science
|April 16, 2025
PubMed
概括

固态纳米孔能够从单细胞中直接分析单分子蛋白质. 这种方法揭示了与纯化样本相比,细胞环境中的不同蛋白质行为,推动了细胞研究.

更多相关视频

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K
A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

7.4K

相关实验视频

Last Updated: Jun 14, 2026

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.5K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K
A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

7.4K

科学领域:

  • 生物物理学的生物物理.
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 在复杂的细胞样本中直接分析蛋白质对于理解细胞多样性和疾病至关重要.
  • 当前的方法经常与细胞环境的复杂性和异质性作斗争.
  • 单分子分析为蛋白质研究提供了更高的灵敏度和分辨率.

研究的目的:

  • 探索化 (SiN) 固态纳米孔的潜力,直接从复杂的细胞样本中进行单分子蛋白质分析.
  • 开发和验证一种在纳米孔内增强蛋白质捕获和分析的方法.
  • 为了研究从单细胞直接分析的蛋白质与纯化样本之间的差异.

主要方法:

  • 设计了一个纳米孔电泳驱动蛋白与模型蛋白LOV2融合,以提高捕获效率.
  • 利用基于玻璃纳米管的单细胞提取来隔离细胞内容.
  • 使用SiN固态纳米孔进行了ex situ单细胞蛋白质分析,以识别和监测蛋白质构造变化.

主要成果:

  • 通过使用SiN纳米孔成功识别和监测单细胞提取物中LOV2蛋白的构造变化.
  • 通过工程驱动蛋白来证明目标蛋白质的增强捕获效率.
  • 与纯化蛋白质样本相比,直接从单细胞中分析时观察到蛋白质特征的显著差异.

结论:

  • 固态纳米孔是单细胞,单分子蛋白质分析的强大工具.
  • 这种技术提供了对蛋白质动态和相互作用的洞察,这些蛋白质在它们的原生细胞环境中相互作用.
  • 这些发现为研究分子层面的细胞异质性和疾病机制开辟了新的途径.