40个R2R3-MYB的特异性景观揭示了paralogs如何通过同位体结合来准不同的cis元素
Tian Li1, Hao Chen1, Nana Ma1,2
1Haixia Institute of Science and Technology, National Engineering Research Center of JUNCAO, College of JUNCAO Science and Ecology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology Fujian Agriculture and Forestry University Fuzhou China.
iMeta
|April 16, 2025
概括
在R2R3-MYB转录因子 (TFs) 中的同质化改变了DNA基因的识别,从而实现了明显的TF结合. 这项研究揭示了TF二元化如何改变约束特异性,引导TF到独特的监管站点.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 植物科学 植物科学
背景情况:
- 类似的转录因子 (TFs) 经常结合类似的DNA动机.
- 同体化是TF歧视的一个已知的机制.
- 在改变密切相关的TFs的DNA模式识别方面,同质化作用的理解仍然较少.
研究的目的:
- 调查同质化如何影响R2R3-MYB转录因子中的DNA图案识别.
- 描述VIII亚族R2R3-MYBs.的特异性景观.
- 为了确定指导密切相关的TF到特定的cis-regulatory网站的替代机制.
主要方法:
- 使用高通量SELEX (指数式丰富对联体的系统演化) 来分析TF DNA结合特异性.
- 833个图案模型是从SELEX数据中策划的.
- 进行了单体和二元结合特异性的比较分析.
主要成果:
- 表明同位体化直接改变了R2R3-MYB TFs中的DNA图案识别.
- 对40个VIII亚系R2R3-MYBs的特异性分析显示,在同位素结合时发生了显著的变化.
- 在同质化后,AtMYB2识别出独特的cis调节序列,与相关的CCWAA盒AtMYBs不同.
结论:
- 同位体化提供了一个通过修改它们的DNA结合偏好来区分密切相关的TF的替代机制.
- 这项研究提供了关于VIII亚族R2R3-MYBs的序列偏好的广泛数据.
- 修改过的AtMYB2二维特异性准了参与特定转录激活的进化保守的基因组位点.
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