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相关概念视频

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.6K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.6K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

592
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
592
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

557
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
557

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相关实验视频

Updated: May 13, 2025

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

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关于NMR代谢学最佳实践:当前状态

Robert Powers1, Erik R Andersson2, Amanda L Bayless3

  • 1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.

Trends in analytical chemistry : TRAC
|April 16, 2025
PubMed
概括

核磁共振代谢学研究往往缺乏必要的细节和标准化的报告. 本次调查强调了在进行和报告这些科学研究方面需要最佳实践.

关键词:
最好的做法 最好的做法文献调查 文献调查代谢学 代谢学 代谢学这是NMR的NMR.

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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
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Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
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相关实验视频

Last Updated: May 13, 2025

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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
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科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 生物信息学是一种生物信息学.

背景情况:

  • 核磁共振 (NMR) 代谢学是研究生物系统的强大工具.
  • 对NMR代谢学研究的标准化报告对于可重复性和数据解释至关重要.
  • 之前的评估表明,有关代谢学实验的报告存在差异.

研究的目的:

  • 调查当前进行和报告NMR代谢学研究的实践.
  • 发现报告实验基本细节和统计方法的漏洞.
  • 鼓励开发和采用该领域的最佳实践.

主要方法:

  • 从PubMed.Med.来源,对2020年的463篇论文和2010年的80篇论文进行文献调查.
  • 研究人员手动分析选定的论文,以评估报告的实验程序和协议的完整性.
  • 评估统计方法和模型验证实践.

主要成果:

  • 许多研究报告的实验细节和统计方法不完全.
  • 监督多变量统计分析的不正确应用和缺乏验证是常见的.
  • 协议和软件的高度多样性表明缺乏共识和标准化的做法.

结论:

  • 目前的NMR代谢学研究在方法和报告方面表现出相当大的异质性.
  • 对于NMR代谢学界来说,迫切需要建立和遵守共同的标准.
  • 采用最佳实践将提高代谢学数据的可靠性和可比性.