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高速离心法未能减轻D-二分体测量中的严重脂质干扰

Xiaoping Xu¹, Shuqian Cai¹, Junchi Xue¹

¹Department of Clinical Laboratory, Jinhua Municipal Central Hospital, Jinhua, China.

International journal of laboratory hematology

|April 16, 2025

概括

高速离心法无法准确测量脂质样本中的D-二分体,这可能导致误诊. 直接稀释可能是一个更好的方法来管理脂质干扰在D-二元测试.

科学领域:

临床化学临床化学
实验室医学实验室医学
血液学血液学血液学

背景情况:

脂质干扰在实验室测试中是一个常见的挑战.
精确的D-二次数测量对于诊断血栓状况至关重要.

研究的目的:

为了评估脂质度 (LT) 对D-二次体测量的影响.
在脂质样本中比较常规离心法 (RC) 和高速离心法 (HC).

主要方法:

脂质样本通过LT进行分级,并分析凝血参数.
在RC和HC之后,测量了D-二聚体和其他凝血指数.

主要成果:

在HC后的严重LT样本 (3-4级) 中,D-二聚合物偏差率超过50%.
HC未能解决干扰,D-二聚体附着在脂质分数上.
脂质层中的D-二聚物水平明显高于血层.

结论:

在严重的脂质样本中,HC并不能减轻D-二元体的度干扰.
这种干扰可能导致血栓状况的误诊.
直接稀释可能是一个可行的替代方案,用于精确的D-二次元测量在脂质样本.

关键词:

D-二聚合物 D-二聚合物凝血是凝固的过程.高速离心方式的离心法.脂血症是什么意思脂血症是什么意思

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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...