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通过双重质谱测量检测红细胞蛋白抗原

Sandip Chavan¹, Ramanath Majumdar¹, Neha Joshi^1,2

¹Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, Minnesota, USA.

|April 16, 2025

概括

双重质谱 (MS/MS) 显示出检测常见红细胞 (RBC) 抗原的前景. 虽然MS/MS确定了几个血型系统的,但临床RBC表型化需要进一步改进.

科学领域:

蛋白质组学是指蛋白质组学.
免疫遗传学免疫遗传学
临床诊断临床诊断临床诊断

背景情况:

协奏质谱法 (MS/MS) 在识别蛋白质变异方面表现出色,类似于临床上显著的红细胞 (RBC) 抗原.
红细胞抗原对于输血兼容性和疾病诊断至关重要.

研究的目的:

评估MS/MS在检测主要血液组系统中常见的红细胞抗原方面的疗效.
探索MS/MS作为RBC抗原的潜在临床表型模式.

主要方法:

使用非向性和向性液体染色学-MS/MS (LC-MS/MS) 分析剩余的献血样本.
蛋白酶对红细胞膜蛋白的消化,以识别特定的.
使用LC-选择反应监测 (LC-SRM) 检测含有抗原的.

主要成果:

非向LC-MS/MS成功检测了大多数向红细胞抗原的蛋白特异性,对3带阴离子运输蛋白,基底细胞粘附分子,Kell和甘氨酸A的高覆盖率.
向LC-SRM证实了Cw,Dia/Dib,Kpb和Wra/Wrb的抗原特异性,与之前的血清学和基因造型结果保持一致.
不针对性的LC-MS/MS没有检测到甘氨酸B.

结论:

多发性硬化症/多发性硬化症可以检测来自多个血型系统的,显示出红细胞抗原表型化的潜力.
目前的MS/MS方法可以识别常见的红细胞抗原的一个子集,但需要进一步优化临床应用.
在样本准备和MS/MS检测灵敏度的改进是必要的,以获得广泛的临床采用.

关键词:

红细胞输血红细胞输血免疫血液学 (红细胞血清学,血型)输血服务业务的运营.

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