相关实验视频

Updated: May 23, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

细菌酸盐生产氧化Fe(II) 生物修复废弃煤矿排水中的酸性废弃煤矿排水

Anna Vietmeier^1,2, Michelle Valkanas³, Natalie Lamagna⁴

¹Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, USA.

Applied and environmental microbiology

|April 16, 2025

概括

煤矿排水整治中的细菌可以使用酸盐的减少产生酸盐,然后化学氧化铁. 这种依赖酸盐的铁氧化 (NDFO) 过程在被动修复系统中很常见,它提供了一种从酸性矿井排水中去除铁的新方法.

科学领域:

环境微生物学环境微生物学
生物地质化学生物地质化学
环境工程环境工程

背景情况:

被动整治系统 (PRS) 处理废弃的煤矿排水 (AMD).
微生物铁氧化是AMD修复的关键,特别是在酸性条件下.
驱动非生物铁氧化的生物反应的理解较少.

研究的目的:

在酸性AMD中研究依赖酸盐的铁氧化 (NDFO).
识别能够NDFO的细菌,并了解它们在铁修复中的作用.
探索NDFO在酸性AMD中增强铁去除的潜力.

主要方法:

从酸性PRS中选4,560种培养细菌,以检测铁氧化和NDFO.
隔离和识别能够产生NDFO的细菌,包括Paraburkholderia spp.
细菌基因表达 (napA) 和代谢物产生 (酸盐) 的分析.
16S rRNA基因测序以评估细菌群体的组成.

主要成果:

在所有池中,在2.1%11.4%的可培养细菌中观察到铁氧化.
在1.4%6.0%的可培养细菌中检测到NDFO.
五个NDFO分离物被确定为Paraburkholderia spp.,其中隔离物AV18显示与铁氧化 (NRIO) 相关的酸盐产量.
编码酸盐减少酶的napA基因在酸盐生产过程中被AV18表达.
在所有PRS池中,Burkholderiales是丰富的.

结论:

细菌酸盐降解为酸盐是酸性AMD中铁氧化的一个重要驱动因素.
酸盐还原-铁氧化 (NRIO) 途径是酸性条件下铁去除的普遍机制.
产生酸盐的细菌,特别是Paraburkholderia,代表了一个有希望的,但尚未充分利用的资源,用于改善酸性AMD中的铁生物修复.

关键词:

废弃矿井排水系统 (AMD)生物地化学循环的循环.生物修复生物修复铁氧化铁的氧化过程减少酸盐的减少酸盐还原氧化铁氧化 (NRIO)酸盐依赖性铁氧化 (NDFO) 是一种铁氧化过程.被动修复系统 (PRS) 是一种被动修复系统.

更多相关视频

Laboratory Simulation of an IronII-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria

Laboratory Simulation of an IronII-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria

Published on: July 24, 2016

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

相关概念视频

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

Overview of Nitrogen Metabolism

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...

2° Amines to N-Nitrosamines: Reaction with NaNO2

2° Amines to N-Nitrosamines: Reaction with NaNO₂

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.

The Nitrogen Cycle

The Nitrogen Cycle

Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...