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沙拉蔬菜作为抗微生物耐药菌的储备:探索多样性,耐药性,毒性和等离子体动力学
Ihab Habib1,2, Mushtaq Khan3,4, Glindya Bhagya Lakshmi1,2
1Veterinary Public Health Research Laboratory, Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates.
在阿联的沙拉蔬菜中,Enterococcus物种具有有关抗菌素耐药性 (AMR) 和毒性因子. 全基因组测序揭示了移动等离子体上的耐药性基因,突出了食物传播的传播风险.
科学领域:
- 食品微生物学 食品微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
背景情况:
- 菌种类是常见的食源性细菌,可以携带抗微生物耐药性 (AMR) 和毒性基因.
- 沙拉的成分经常被生吃,这可能是传播食物传播病原体的潜在载体.
- 了解新鲜农产品中Enterococcus的AMR和毒性概况对于公共卫生至关重要.
研究的目的:
- 调查阿联沙拉蔬菜中Enterococcus物种的流行率,AMR模式,毒性因子和等离子体组成.
- 使用全基因组测序 (WGS) 识别特定的耐药性和病毒性基因.
- 评估耐药性和毒性Enterococcus菌株的食物传播潜力.
主要方法:
- 在10个月内收集的400个沙拉蔬菜样本中隔离和识别Enterococcus物种.
- 孤立的Enterococcus菌株的抗微生物敏感性测试 (AST).
- 选择的分离物的全基因组测序 (WGS) 来分析AMR基因,毒性因子和等离子体含量.
主要成果:
- 在沙拉蔬菜中检测到Enterococcus的高检测率 (85.5%),其中E. casseliflavus,E. faecium和E. faecalis是主导物种.
- 55.3%的分离物体对至少一种抗微生物药物产生了耐药性,18.8%的分离物体对多种药物产生了耐药性 (MDR).
- 在一个易受万科胺素感染的E. faecalis中,WGS确定了万科胺素耐药性基因 (vanC,vanXY-C2),以及四环素,化物和氧化丁耐药性基因. 检测到病毒性因素,包括细胞解酶集群. 可调动的等离子体经常携带AMR基因.
结论:
- 在阿联,沙拉蔬菜作为Enterococcus菌株的储存库,具有临床相关的AMR决定因素和毒性特征.
- 移动等离子体上存在抗药性基因表明,水平基因转移和传播的风险很高.
- 加强食品传播的抗菌素耐药性监测,包括WGS,对于控制食物链中耐药性肠球菌的传播至关重要.
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