草多糖的制备,表征和生物活性
Libo Wang1, Yumeng Zhao1, Junwen Liu1,2
1College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.
Foods (Basel, Switzerland)
|April 16, 2025
概括
降解的草多糖化物 (DSFP-500和DSFP-700) 表现出增强的物理化学特性和强大的抗氧化和抗补充活性. 这些发现突出了它们在功能性食品和制药开发中的应用潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 多糖是复杂的碳水化合物,具有多样化的生物活动.
- 草果多糖化物 (PSP) 在各种应用中显示出潜力.
- 修改PSP结构可以改变它们的特性和生物活性.
研究的目的:
- 通过超声波降解获得的两个降解的草果多糖体DSFP-500和DSFP-700的特征.
- 评估它们的物理化学性质,包括分子量,单糖组成,热稳定性,水容量 (WHC) 和油容量 (OHC).
- 评估它们在体外的抗氧化和抗补充活性.
主要方法:
- 超声波降解原生草多糖化物 (PSP).
- 分子重量,单糖化合物组成,热稳定性,WHC和OHC的表征.
- 在体外评估自由基清除活性 (ABTS+•测试).
- 通过经典和替代途径评估抗补充活性.
主要成果:
- DSFP-500 (809 kDa) 和DSFP-700 (791 kDa) 的分子重量明显低于原生PSP (9479 kDa).
- 这两种降解的多糖都表现出良好的热稳定性,WHC (5.535.70 g/g) 和OHC (9.289.34 g/g).
- DSFP-500和DSFP-700表现出强烈的自由基清除活动 (52.0655.97%在4.0毫克/毫升) 和显著的抗补充活动,DSFP-700更强.
结论:
- 超声波降解有效地降低了草多糖的分子量,同时保持或增强了可取的物理化学特性.
- 降解的多糖类具有显著的抗氧化和抗补充活性,表明它们的实用性.
- 作为食品和制药行业的功能成分,DSFP-500和DSFP-700显示出有前途的潜力.
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