核桃蛋白与EGCG的快速共价结合:揭示了超声波辅助的比例优化,结合机制和结构功能转换
Yuanyuan Wei1, Liping Sun1, Ying Gu1
1Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Foods (Basel, Switzerland)
|April 16, 2025
概括
超声波辅助的性处理改善了核桃蛋白分离物 (WPI) 和表甲基-3-酸盐 (EGCG) 复合物,提高了功能性食品应用的溶解性和乳化.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 核桃蛋白分离物 (WPI) 和多的应用往往因溶解性差而受到阻碍.
- 开发稳定的蛋白质多复合体对于提高其功能至关重要.
研究的目的:
- 研究性和超声波辅助性处理对核桃蛋白分离物 (WPI) 和表甲基-3-酸盐 (EGCG) 共价复合物的作用.
- 描述这些新型蛋白质多复合物的结构和功能性质.
主要方法:
- 制备WPI-(-) -epigallocatechin-3-gallate (WPI-EGCG) 和超声波辅助的WPI-EGCG (UWPI-EGCG) 共价复合物.
- 使用富里埃变换红外光谱学和光谱学进行分析,以评估结构变化.
- 粒子大小的表征,泽塔潜力,多结合性,溶解性和乳化性质.
主要成果:
- EGCG与WPI的共价结合改变了蛋白质的二级和三级结构,增加了随机线圈含量.
- 在UWPI-EGCG样本中,粒子大小减少 (153.67 nm) 和增高的泽塔电位 (25.4 mV).
- UWPI-EGCG复合物显示出优越的多结合能力 (53.37 ± 0.33 mg/g蛋白),以及出色的溶解性和乳化.
结论:
- 超声波辅助性处理是一种有效的方法,可以创建稳定和功能性的核桃蛋白-多复合体.
- 这些增强复合物显示出在功能性食品产品中使用的巨大潜力.
- 这项研究提供了一个有前途的策略,以改善核桃蛋白质隔离剂在食品工业中的应用.
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