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大豆蛋白水解酸的潜在功能是诱导C2C12细胞的肌体差异化

Yinglei Chen¹, Changwu Xiong¹, Yingzhi Wan¹

¹Hubei Provincial Key Laboratory of Yeast Function, Angel Yeast Co., Ltd., Yichang, China.

|April 16, 2025

概括

大豆蛋白水解剂 (SPH) 有效地促进肌肉卫星细胞的生长和分化,用于培养肉. 这种植物性添加剂显示出作为一个具有成本效益的血清替代品的潜力,促进可持续的食品生产.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
可持续的粮食生产可持续的粮食生产

背景情况:

肌肉卫星细胞 (MSC) 技术是培养肉 (CM) 的关键,为传统农业提供了可持续的替代方案.
细胞培养基的高成本和对胎儿牛血清 (FBS) 的依赖是CM生产的重大障碍.
正在探索基于植物的替代品,以降低成本并提高CM的可持续性.

研究的目的:

评估大豆蛋白水解剂 (SPH) 作为一种潜在的血清替代品,用于增强MSC的扩散和CM的差异化.
调查SPH PU041对C2C12小鼠细胞系的影响,用于肌肉生物学和CM发育的模型.

主要方法:

培养的C2C12细胞具有不同度的SPH PU041和FBS.
评估了细胞的增殖和延长.
免疫光和流细胞测量被用于分析关键肌原性标记物 (MyHC,MyoD).
使用定量实时PCR (qPCR) 来测量肌体发生相关基因 (MyHC,MyoD,MyoG) 的mRNA表达.

主要成果:

SPH PU041显著促进了C2C12细胞的增殖和延长,在0.5g/L时获得最佳结果.
PU041增加了肌重链 (MyHC) 和肌分化因子1 (MyoD) 的蛋白质水平.
qPCR分析证实了PU041在上调MyHC,MyoD和肌原蛋白 (MyoG) mRNA中的作用,表明诱导肌原蛋白分化.

结论:

SPH PU041在培养肉类生产中显示出作为一个具有成本效益的血清替代品的潜力.
这一发现支持开发更可持续,更道德的食品生产方法.
需要进一步的研究来阐明PU041影响肌体分化的确切机制.

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