通过集成的动态代谢学和转录学揭示了对酸盐污染引起的鱼 (Scophthalmus maximus) 肠道功能障碍的见解
Jiachen Yu1, Suyue Zhou1, Ziyi Zhang1
1Laboratory of Pathology and Immunology of Aquatic Animals/Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China.
Aquatic toxicology (Amsterdam, Netherlands)
|April 16, 2025
概括
酸盐污染会影响鱼的肠道代谢和蛋白质吸收. 这项研究揭示了鱼肠道因酸盐暴露而发生的分子变化,影响了氨基酸和能量代谢.
科学领域:
- 水生物质毒理学研究
- 环境科学环境科学
- 鱼类生物学 鱼类生物学
背景情况:
- 酸盐污染对水生生态系统和海洋生物构成全球威胁.
- 鱼肠中酸盐毒性的分子机制尚不清楚.
研究的目的:
- 为了研究鱼肠道中酸盐毒性的分子机制.
- 使用多种omics方法探索对酸盐暴露的代谢反应.
主要方法:
- 鱼被暴露在酸盐 (200 mg/L NO3-N) 中,分别为0,10,20和30天.
- 在肠道组织上进行了综合代谢学和转录学分析.
- 使用了代谢物通路分析 (MetPA) 和短时间序列表达式矿工 (STEM).
主要成果:
- 酸盐暴露显著改变了肠道代谢物网络和代谢受损.
- 关键受影响的途径包括氨基酸代谢 (例如, fenylalanine, tyrosine, tryptophan 生合成) 和能量代谢 (碳水化合物和脂质).
- 网络分析确定了七种涉及的必需氨基酸,并揭示了蛋白质消化,原合成和细胞外矩阵组成中的干扰.
结论:
- 持续的酸盐压力通过干扰蛋白质消化,吸收和ECM完整性来破坏鱼的肠道平衡.
- 这项研究提供了关于鱼类中酸盐毒性机制的关键见解.
- 这些发现可以为改善海洋环境中酸盐管理的战略提供信息.
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