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使用Oligo-LiveFISH进行高分辨率的染色体DNA通信动态成像

Yanyu Zhu1, Ashwin Balaji2, Mengting Han1

  • 1Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.

Cell
|April 16, 2025
PubMed
概括

通过Oligo-LiveFISH,可以在活细胞中实时跟踪特定的DNA区域. 这种新方法可视化了动态基因组组织, 对于了解细胞功能和疾病至关重要.

关键词:
通过CRISPR成像进行DNA通信有机鱼类动态跟踪增强剂与促进剂的相互作用高分辨率成像在线DNA成像不重复的基因组成像聚合物建模主要细胞

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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