进化见解和多I:C诱导的表达变化以及Miichthysmiiuyuyc中的il17ra和il17rc的m6A修饰
Ruotong Tian1, Shang Geng1, Xing Lv1
1Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Fish & shellfish immunology
|April 16, 2025
概括
N6-甲基氨酸 (m6A) 的修改调节了千百万个croaker中的互白素-17受体A (IL17RA) 和IL17RC. 这些修改对抗病毒免疫力至关重要,影响基因表达和mRNA传输.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 介素-17受体A (IL17RA) 和IL17RC是IL-17A信号通路的关键组成部分,对于T辅助细胞17 (Th17) 介导的免疫反应至关重要.
- IL-17受体家族在免疫中的作用,特别是关于像N6-甲基氨酸 (m6A) 这样的表观遗传修饰,仍然在很大程度上未被探索.
研究的目的:
- 为了研究IL17RA和IL17RC在miichthys miiuycroaker (Miichthys miiuy) 的存在和功能.
- 在抗病毒免疫的背景下,探索IL17RA和IL17RC上的m6A修改的调控作用.
主要方法:
- 生物信息学分析以识别和研究il17ra和il17rc基因的进化和结构保护.
- 甲基化RNA免疫沉降测序 (MeRIP-seq) 和MeRIP-PCR用于检测il17ra和il17rc上的m6A修饰.
- 通过多种刺激来评估基因表达和m6A水平.
- 用甲基化抑制剂cycloleucine (CL) 治疗,以评估其对基因表达和mRNA传输的影响.
主要成果:
- 在 miiuy croaker 中发现了IL17RA和IL17RC,显示出与免疫功能相关的进化和结构性保护.
- 在il17ra和il17rc.的终端前体上检测到强大的m6A修饰.
- 聚I:C刺激提高了il17ra和il17rc的表达,同时降低了它们的m6A修饰水平,这表明在抗病毒反应中发挥了作用.
- 环环氨酸 (CL) 没有影响il17ra和il17rc的表达,但增强了il17ramRNA的核到细胞质传输.
结论:
- m6A的修改对IL17RA和IL17RC的功能起着重要的调节作用.
- 这些表观遗传修改与抗病毒免疫反应有关,影响基因表达和mRNA定位.
- 这些发现为m6A表体转录和IL-17受体介导免疫之间的相互作用提供了新的见解.
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