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Updated: May 12, 2025

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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链体的高通量优化,用于将功能蛋白与GFP融合

Gaili Cao1, Zhong Li1, Zhaoguan Wang1

  • 1School of Chemical Engineering and Technology, Tianjin University, Tianjin, China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin, China.

Protein expression and purification
|April 16, 2025
PubMed
概括

为融合蛋白优化链接器对于生物工程至关重要. 这项研究开发了一种新的选方法,以确定绿色光蛋白 (GFP) 融合的高效链接器,增强蛋白质的功能.

关键词:
美国大肠杆菌 (E. coli).光活动的光活性.聚变蛋白质的融合蛋白质是什么链接器工程 链接器工程随机图书馆是一个随机的图书馆.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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