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相关概念视频

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.0K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Transgenic Organisms00:53

Transgenic Organisms

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Overview
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DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.2K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: May 12, 2025

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

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为植物功能基因组学和基因组工程利用可移植的元素.

Xiao-Yuan Tao1, Shou-Li Feng1, Lu Yuan1

  • 1Xianghu Laboratory, Hangzhou 311231, China.

Trends in plant science
|April 16, 2025
PubMed
概括

可转移元素 (TE) 是植物基因组进化和作物化的关键驱动因素. 这一审查整合了关于TE功能,监管和生物技术应用的知识,用于未来的作物改进.

关键词:
互联调节模块 (CRM) 是一个农作物育种 植物育种提高作物的作物改善.基因组的演化 基因组的演化新功能化的新功能化.没有编码的RNAs.转换一个转换器.

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CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

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A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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相关实验视频

Last Updated: May 12, 2025

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CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
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科学领域:

  • 植物基因组学 植物基因组学
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 可转移元素 (TE) 占植物基因组的很大一部分.
  • 转基因会影响基因表达,基因组进化和作物化.
  • 现有的TE知识需要对生物技术应用进行全面的整合和探索.

研究的目的:

  • 提供一个彻底的概述,连接植物TE的发现,机制和技术.
  • 讨论TE的识别,功能和表观遗传调节.
  • 突出在功能性基因组学和基因组工程中使用活跃的TE.

主要方法:

  • 对TEs的功能基因组学研究的审查.
  • 对管理TE的表观遗传调节机制的分析.
  • 检查活跃TE的生物技术应用.

主要成果:

  • 功能性基因组学方法已经推进了对TE识别和功能的理解.
  • 表观遗传调节在控制TE活动方面发挥着至关重要的作用.
  • 活跃的TE为植物功能基因组学和基因组工程提供了有希望的工具.

结论:

  • 整合对TEs的知识对于推动植物科学的发展至关重要.
  • 了解TE机制和应用将有利于作物育种.
  • 对未来的植物合成生物学来说,TE具有显著的潜力.