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在骨质疏松症中与氧化酸化相关的差异表达基因的生物信息学分析

Songmao Wang^1,2, Yaling Wang³, Minfeng Gan⁴

¹Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

European journal of medical research

|April 16, 2025

概括

这项研究确定了10个关键的氧化酸化 (OXPHOS) 相关基因,在骨质疏松症 (OP) 中差异地表达. 这些基因,包括VPS35和TBC1D2,显示出作为OP诊断生物标志物和治疗点的潜力.

科学领域:

生物化学生物化学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

骨质疏松症 (OP) 是一种代谢性骨病,其特征是骨质减少和骨折风险增加.
新出现的证据表明氧化酸化 (OXPHOS) 在OP病变发生过程中.
这项研究调查了OP中OXPHOS相关基因的差异表达和功能作用.

研究的目的:

在骨质疏松症中识别差异表达的OXPHOS相关基因.
探索这些基因的功能意义和潜在诊断价值.
为了揭示涉及OXPHOS的OP背后的分子机制.

主要方法:

从公共骨质疏松症数据集下载和分析基因表达数据 (GSE56815,GSE7429).
整合了OXPHOS基因组,并进行了差异表达分析,功能丰富 (GO,KEGG) 和GSEA.
进行了相关性,蛋白质-蛋白质相互作用 (PPI) 网络,mRNA-miRNA,mRNA-TF相互作用和免疫透分析. 在体外验证基因表达.

主要成果:

在骨质疏松症中鉴定出10个不同表达的OXPHOS相关基因 (OXPHOSRDEGs),包括VPS35和TBC1D2.2.
功能性丰富显示出参与蛋白质代谢和内细胞突变途径.
VPS35和TBC1D2的诊断准确度很高 (AUC分别为0.832和0.751).
在PPI网络分析中,发现了8个具有功能相似性的枢纽基因.

结论:

这项研究系统地阐明了使用生物信息学在OP中OXPHOS相关的基因表达和机制.
已识别的关键基因为OP的分子基础提供了洞察力.
这些基因代表了潜在的诊断生物标志物和骨质疏松症的治疗点.

关键词:

生物标志物生物标志物微分表达式分析微分表达式分析骨质疏松症是一种骨质疏松症.氧化酸化是一种氧化酸化.治疗目标治疗目标

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