相关实验视频

Updated: May 11, 2025

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

ATG9A控制着自细胞生物生成的所有阶段

Ruheena Javed^1,2, Muriel Mari³, Einar Trosdal^1,2

¹Autophagy, Inflammation and Metabolism Center of Biomedical Research Excellence, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.

|April 17, 2025

概括

与自相关的9A (ATG9A) 蛋白,以前以启动自细胞形成而闻名,也指导其关闭. ATG9A通过IQGAP1与CHMP2A合作,协调整个自细胞生物发生过程.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
自学研究自学研究

背景情况:

自是一种基本的细胞过程,用于降解和回收细胞组件.
自细胞形成是宏自的一个标志,涉及到连续的步骤:启动,扩张和关闭.
ATG9A (与自相关的9A) 已因其在自细胞启动和扩张中的作用而得到认可.

研究的目的:

为了研究哺乳动物ATG9A在自细胞生物发生中的以前不被重视的功能.
阐明ATG9A在自细胞形成的最后关闭阶段的作用.
为了确定ATG9A的相互作用伙伴参与自细胞关闭.

主要方法:

同免疫沉 (Co-IP) 测试以确定蛋白质相互作用.
高含量显微镜 (HCM) 用于可视化自细胞形成.
使用淘汰赛 (KO) 模型和特定分子相互作用,分析ATG9A在自细胞关闭中的作用.

主要成果:

哺乳动物ATG9A在自细胞形成的闭合阶段 (Z) 中发挥着关键作用.
通过IQGAP1.1,ATG9A直接与CHMP2A合作,这是运输 (ESCRT) -III组件所需的关键内体组分类综合体,通过IQGAP1.
这种相互作用促进了自细胞的最终密封.
ATG9A充当一个中心枢纽,协调自细胞膜生物发生的所有阶段.

结论:

ATG9A在指导自细胞关闭方面具有以前未知的功能.
通过通过IQGAP1.1.与ESCRT-III通道相互作用,ATG9A集成了膜组装和关闭机制.
ATG9A是一种独特的,核心的ATG因子,对完整的自细胞生物发生路径至关重要.

关键词:

自自是一种自的过程.这是ESCRT的特色.在这个问题上,IQGAP IQGAP.线粒细胞衰变 (mitophagy) 是一种神经衰变的过程.在法哥中,法哥是最重要的.结核病是一种肺结核病.

更多相关视频

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

相关概念视频

Autophagy

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...

Delivery Pathways to the Lysosome

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

Autophagic Cell Death

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...

Coat Assembly and GTPases

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...

Export of Misfolded Proteins out of the ER

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...

Rab Cascades

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.