产卵母的十二指肠刷边缘膜内源性粘膜酸酶的表征
Anna Hanauska1, Vera Sommerfeld1, Margit Schollenberger1
1Institute of Animal Science, University of Hohenheim, Stuttgart, Germany.
Frontiers in physiology
|April 17, 2025
概括
粘膜酸酶有助于分解植物酸盐. 酶活性随着年龄的增长而增加,这表明年长的母更好地降解料花酸盐,影响营养的可用性.
科学领域:
- 动物营养 动物营养
- 生物化学 生物化学
- 胃肠病学 胃肠病学
背景情况:
- 的粘膜酸酶可以部分降解食中的植物酸盐.
- 关于这些酶的特性,降解产物和与年龄有关的影响的信息有限.
- 了解这些酸酶对于改善家禽的营养利用至关重要.
研究的目的:
- 在两个产卵母菌株中对内源性粘膜酸酶进行鉴定.
- 研究年龄和饮食中的矿物对这些酶的影响.
- 在十二指甲膜粘膜中识别植物酸盐 (InsP6) 降解产物.
主要方法:
- 两个产卵母品种 (罗曼布朗-经典和罗曼LSL-经典) 接受了含有或不含矿物的食.
- 从19周和24周年龄的母收集了十二指肠粘膜.
- 刷边缘膜 (BBM) 被分离用于酸酶活性测定和体外植物酸盐降解研究.
主要成果:
- 酵素活性和植物酸盐降解没有受到菌株或饮食的显著影响.
- 与19周的母相比,24周的母的酸酶活性显著更高.
- 植物酸盐降解产品表明,十二指甲状腺粘膜中具有主要的6 - 植物酶和次要的5 - 植物酶活性.
结论:
- 年龄显著影响的粘膜酸酶活性,年长的母表现出更高的活性.
- 该研究描述了InsP6降解产品的特征,表明了十二指甲状腺刷边缘膜中特定的植物酶活动.
- 小肠度在调节粘膜酶活性方面可能比食中的作用更为重要.
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