长非编码RNAANRIL/p65负反循环保护炎症性肠病中的肠壁功能
Keqi Yu1, Hong Peng1, Zhechuan Zhang1
1Department of Gastroenterology, Bishan Hospital of Chongqing Medical University, Chongqing, 402760, China.
Non-coding RNA research
|April 17, 2025
概括
长非编码RNAANRIL在炎症性肠病 (IBD) 中降低调节,恶化肠道屏障功能. 恢复ANRIL水平通过抑制NF-κB通路来保护肠道屏障,为IBD提供了潜在的治疗策略.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 炎症性肠病 (IBD) 的特点是肠道屏障功能受损.
- 长非编码RNAs (lncRNAs) 参与IBD的发病,可能会影响屏障的完整性.
- 在IBD相关的屏障功能障碍中,lncRNA ANRIL的特定作用仍然在很大程度上未被探索.
研究的目的:
- 研究下调的lncRNAANRIL在IBD中调节肠道屏障功能的作用.
- 阐明ANRIL影响肠道屏障完整性的潜在分子机制.
- 在IBD模型中探索ANRIL的治疗潜力.
主要方法:
- 对IBD患者ANRIL表达的分析以及与疾病严重程度的相关性.
- 在硫酸 (DSS) 诱导的小鼠结肠炎模型中,ANRIL的过度表达.
- 转录组测序以确定受ANRIL影响的途径.
- 使用细胞和动物模型研究ANRIL/NF-κB信号轴.
- 对ANRIL与YY1和p65.5的相互作用的评估.
主要成果:
- 在性结肠炎 (UC) 患者中,ANRIL的表达显著下调,与疾病进展相关.
- 在DSS诱导的大肠炎小鼠中,ANRIL的过度表达减少了结肠损伤,抑制了促炎性细胞因子 (IL-6,TNF-α,IL-1β),并改善了肠道屏障功能.
- 在小鼠大肠炎和LPS诱导的细胞模型中,ANRIL过度表达抑制了NF-κB通路激活.
- 安瑞尔具有竞争性地结合YY1,抑制YY1-p65相互作用和随后的NF-κB转录活性.
- 确定了一个负反循环,p65结合ANRIL促进子,增强ANRIL表达.
结论:
- 在IBD中,lncRNA ANRIL在维持肠道屏障功能方面发挥着保护作用.
- 通过与YY1和p65.5相互作用,ANRIL通过抑制NF-κB信号通路来改善炎症和屏障缺陷.
- ANRIL/p65负反循环为IBD治疗提供了一个有希望的治疗目标.
更多相关视频
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
12.2K
07:32An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
Published on: December 3, 2020
5.7K
相关概念视频
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.0K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K
01:23Renewal of Intestinal Stem Cells
2.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.5K
01:28Inflammatory Response
1.6K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.6K
02:39lncRNA - Long Non-coding RNAs
8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
02:15Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K