相关实验视频

Updated: May 11, 2025

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

空间对比度计算的数值实现的选择会影响激光光斑对比度成像中的微循环量化

Marc Chammas¹, Frédéric Pain¹

¹Université Paris-Saclay, Institut d'Optique Graduate School, CNRS, Laboratoire Charles Fabry, Palaiseau, France.

Journal of biomedical optics

|April 17, 2025

概括

精确的血液流量指数计算对于激光斑点对比成像 (LSCI) 是至关重要的. 不同的空间对比计算方法显著影响流量定量,MESI模型显示的精度高于LSCI近似值.

科学领域:

生物医学光学生物医学光学
微循环成像技术的成像技术
量化流量分析是一种量化流量分析.

背景情况:

激光光斑对比成像 (LSCI) 是一种用于可视化微循环的非侵入性技术.
精确计算空间对比度对于得出LSCI中的血液流量指数至关重要.
现有的文献缺乏对LSCI空间对比计算方法的全面比较.

研究的目的:

评估不同计算方法对从空间对比度获得的血液流量量化的影响.
为了比较在LSCI中计算空间对比度的各种数值方法的准确性.

主要方法:

在Python和ImageJ中使用模拟和体内鼠标数据进行空间对比计算的比较.
使用LSCI异常估计和适应多次曝光斑点成像 (MESI) 模型来估计脱离关系时间.
分析噪音和有限曝光时间对LSCI准确性的影响.

主要成果:

通过LSCI方法,模拟数据显示血液流量指数变化高达58%.
与模拟数据相匹配的MESI模型显示的差异仅为少数百分比.
实验数据显示LSCI近似的相对差异高达35%,而MESI提供了一致的折叠关系时间值.

结论:

必须验证空间对比度计算的数值方法,以准确估计流量指数.
统一的波器方法提供了准确的空间对比值和卓越的计算效率.
与LSCI近似相比,MESI导出对方法偏差的敏感性较小,尽管并非完全免疫.

关键词:

2D过器可以使用2D过器.血液的流动,血液的流动.卷积的卷积卷积的卷积激光光斑对比成像对比成像空间对比度空间对比度

更多相关视频

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

Published on: November 11, 2010

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

Published on: January 12, 2019

相关概念视频

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...