评估被动抽样用于监测E.I. 大肠杆菌和Cryptosporidium种类. 在环境水域中
Ilya Law1, Erin Becker1, Brandon S Spoja1
1Water, Health and Applied Microbiology Lab (WHAM Lab), Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
ACS ES&T water
|April 17, 2025
概括
膜被动采样器可以有效地在水中检测大肠杆菌和杆菌,长达96小时. 这种方法对水生环境中的病原体监测具有前景,提供与传统采样相比可靠的结果.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 在水生环境中对病原体的监测对公众健康至关重要.
- 传统的复合采样可能是劳动密集型的,可能会错过间歇性的病原体存在.
- 被动采样为评估水质提供了一个潜在的更有效的替代方案.
研究的目的:
- 为了比较膜被动采样与复合采样在检测大肠杆菌和Cryptosporidium方面的有效性.
- 为了评估这些病原体在不同水矩阵中的被动样本上依赖时间的吸收.
- 确定废水和地表水中的被动采样器的最佳部署时间.
主要方法:
- 使用膜被动采样和复合采样进行了受控实验.
- 废水和地表水样本被分析为大肠杆菌和杆菌.
- 在各种时间点 (4-96小时) 测量了被动样本对病原体的吸收.
主要成果:
- 两种采样方法都在所有时间点的所有样本中检测到大肠杆菌.
- 被动采样显示,Cryptosporidium的检测率与地表水 (31%对41%) 和废水 (76%对86%) 的复合采样相似.
- 在地表水中观察到对被动样本的大肠杆菌和杆菌的线性吸收时间长达96小时,对于大肠杆菌而言,废水吸收时间较早稳定.
结论:
- 膜被动采样器在地表水中检测大肠杆菌和Cryptosporidium的有效时间长达96小时.
- 被动采样为复合采样提供了一个可行的替代方案,用于监测关键的水传播病原体.
- 进一步的研究可以优化各种水生基质和病原体的被动采样协议.
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