使用工程化糖菌 (Saccharomyces cerevisiae) 生产玻利,坎佛和玻利酸
Masahiro Tominaga1,2, Kazuma Kawakami2, Hiro Ogawa2
1Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Metabolic engineering communications
|April 17, 2025
概括
现在,人工酵母可以产生双环单烯,如玻利,甘和玻利酸. 这种微生物生产为传统的不可再生资源提供了一个可持续的替代方案.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 双环单烯是有价值的化合物,在香料和制药中具有应用.
- 当前的生产方法往往依赖于不可持续的石化资源.
- 微生物发酵为单烯合成提供了一个有前途的可持续替代方案.
研究的目的:
- 为微生物生产双循环单烯而设计Saccharomyces cerevisiae.
- 建立一个可持续的生物生产平台,用于醇,甘和乙酸.
- 为了证明和酸的异质生产的可行性.
主要方法:
- 工程化Saccharomyces cerevisiae表达Salvia officinalis bornyl pyrophosphatase合成酶的融合蛋白和突变的S. 谷物菌的酸盐合成酶. farnesyl pyrophosphate synthase. 谷物菌的酸盐合成酶. farnesyl pyrophosphate synthase.
- 从Enterococcus faecalis中引入两个关键的美酸盐路由酶 (乙乙-CoA硫酶/基甲基酸-CoA减少酶和HMG-CoA合成酶).
- 特定酶的共同表达,醇脱酶或乙烯转移酶,以产生花和乙酸.
主要成果:
- 工程酵母在摇瓶发酵过程中产生了高达23.0 mg/L的波醇.
- 通过表达玻利醇脱酶,获得了23.5 mg/L的坎佛的产生.
- 通过表达bornyl乙转移酶,实现了21.1mg/L的博尼尔酸盐的合成.
结论:
- 这项研究介绍了在酵母中首次成功地产生坎佛和酸的异质生产.
- 工程师S.S.的设计 谷菌平台能够可持续地生产有价值的双循环单烯的微生物.
- 这项工作为这些化合物的工业规模生物合成铺平了道路.
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