在康复性肝移植接受者中,SARS-CoV-2引起的明显的免疫记忆
Mengcheng Liu1,2,3, Feng Wu1,2, Binwei Duan1,2
1Department of General Surgery Center, Beijing YouAn Hospital, Capital Medical University, Beijing Institute of Hepatology, Beijing, China.
Frontiers in immunology
|April 17, 2025
概括
从COVID-19感染中恢复过来的肝移植接受者 (LTR) 根据抗体状态显示出不同的T细胞反应. 与nAb-LTRs相比,那些具有中和抗体 (nAb+) 的人具有更强的T细胞激活和记忆回忆.
科学领域:
- 免疫学 免疫学 免疫学
- 移植科学 移植科学
- 病毒学 病毒学
背景情况:
- 了解肝移植接受者 (LTR) 在SARS-CoV-2感染后的T细胞反应是有限的.
- 在没有先前接种疫苗的情况下从COVID-19中恢复的LTR提供了一个独特的模型来研究免疫反应.
- 在LTRs中,T细胞表型和记忆形成的差异对于管理免疫力至关重要.
研究的目的:
- 调查和比较T细胞表型和记忆形成在LTRs中和没有中和抗体 (nAb) 在COVID-19恢复后.
- 在LTR中识别与有效的SARS-CoV-2免疫记忆相关的特定T细胞子集和细胞因子档案.
- 为了告知LTRs的疫苗接种策略和免疫管理.
主要方法:
- 在COVID-19感染后六个月的6个nAb+和6个nAb-LTR中对T细胞子集和细胞因子表达的比较分析.
- 量化抗RBDIgG标位和中和抗体活性.
- 使用SARS-CoV-2 XBB尖峰池进行体外刺激,以评估T细胞反应 (PD-1,CTLA-4,TNF-α,IFN-γ,IL-17A,穿孔素).
主要成果:
- nAb+ LTRs表现出较高的抗RBD IgG标位和中和活性.
- 终端效应体CD8+ - 3细胞在nAb+LTRs下降,而IL-10在nAb-LTRs上升.
- 在体外刺激显示效应记忆CD4+细胞增加,在nAb+LTR中PD-1较低,在nAb-LTR中终端效应CD8+-3细胞中CTLA-4/IL-10较高.
- 两组都显示了具有TNF-α和IFN-γ的SARS-CoV-2特异性T细胞;在nAb+LTR中发现了穿孔素.
结论:
- 在SARS-CoV-2感染后,LTRs开发中和抗体显示出更强大的T细胞反应和免疫记忆.
- 在nAb+和nAb- LTRs之间存在T细胞子集和激活标记的显著差异.
- 这些发现凸显了T细胞免疫评估对优化LTRs疫苗接种和管理策略的重要性.
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