相关实验视频

Updated: May 3, 2026

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

生物记录器和miRNAs是测量运输期间羊羔生理变化的创新工具

Isabella Manenti¹, Irene Viola¹, Francisco Canto²

¹Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.

Journal of animal science

|April 17, 2025

概括

运输牲畜可能会很紧张. 这项研究使用了羔羊的生理和分子标记来确定运输期间的压力时刻,确定卸载是关键压力因素. 循环中的microRNAs显示出在运输过程中评估动物福利的生物标志物的潜力.

科学领域:

动物科学动物科学
兽医医学兽医医学兽医医学
生物标志物生物标志物

背景情况:

牲畜运输涉及许多压力因素,包括处理,养密度和环境因素.
动物对这些运输压力因素的感知和适应尚未完全理解,这使得福利评估变得复杂.

研究的目的:

用生理和分子标记物识别羊羔运输过程中的压力时刻.
评估循环微RNAs (c-miRNAs) 作为运输期间动物福利的生物标志物的潜力.

主要方法:

羊羔配备了生物记录器来记录心率 (HR) 和体温 (BT).
收集唾液和血样本进行皮质醇,肌酸激酶 (CK),乳酸脱酶 (LDH) 和c-miRNA表达分析.
分析了生理和分子数据,以将压力反应与运输事件相关联.

主要成果:

卸载导致HR,皮质醇,CK和LDH显著增加,BT下降.
四个特定的c-miRNAs (miR-23a, -27a, -17, -24) 在卸载后显示出显著的表达变化.
血miR-23a,-24,和-27a的表达与LDH水平正相关.

结论:

卸载是运输期间羊羔的主要压力因素.
绵羊血和唾液c-miRNAs在运输压力下表现出动态变化,表明它们作为福利生物标志物的潜力.
这项研究提供了评估和改善牲畜运输条件的工具.

关键词:

适应应对的适应反应这是一个微型RNA.传感器传感器传感器羊群羊群羊群的羊群是什么意思交通运输运输运输运输运输运输运输运输运输

更多相关视频

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Continuous Telemetric In Utero Tracheal Pressure Measurements in Fetal Lambs

Continuous Telemetric In Utero Tracheal Pressure Measurements in Fetal Lambs

Published on: December 22, 2023

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...