奥里桑醇通过肠道微生物群重编程和TLR4/NF-κB信号抑制改善了小鼠MCD诱导的代谢功能障碍相关的脂肪肝炎
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Updated: May 11, 2025

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
Published on: March 11, 2017
Naqash Alam1, Xinhua Ding1, Yu Fu1
1Laboratory of Animal Center, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.
香醇 (ORY) 通过改善肠道屏障功能和减少炎症,减轻小鼠的代谢功能障碍相关的脂肪肝炎 (MASH). ORY积极调节肠道微生物群,为MASH的预防和治疗提供了潜力.
科学领域:
- 肝病学和胃肠病学 肝病学和胃肠学
- 微生物组研究 微生物组研究
- 营养学科学 营养学科学
背景情况:
- 代谢功能障碍相关的脂肪肝炎 (MASH) 影响全球四分之一的人口,肠道微生物群的相互作用在其发病过程中发挥着关键作用.
- 香醇 (ORY) 是一种大米的化合物,具有已知的抗氧化,抗炎和代谢调节性质.
研究的目的:
- 调查奥里桑醇 (ORY) 对改善MASH的疗效.
- 探索ORY补充剂,肠道微生物群调节和MASH进展之间的关联.
主要方法:
- 雄性C57BL/6J小鼠使用甲素-胆缺乏的饮食诱导MASH,并补充ORY (300 mg/kg/day) 或控制4周.
- 对肝损伤,炎症标志物,脂质积累和TLR4/NF-κB信号的评估.
- 使用16S rDNA测序来分析肠道微生物群的组成和多样性.
主要成果:
- 在MASH小鼠中,ORY显著降低了肝酶,脂质积累和组织学损伤,同时恢复了肠道屏障的完整性.
- ORY降低了血脂聚糖 (LPS) 和炎症性细胞因子,降低了肝脏TLR4/NF-κB信号的调节,并增强了肠道紧密结合蛋白 (ZO-1,ocludin).
- 微生物群分析显示ORY增加了有益细菌 (例如,乳酸菌,阿克尔曼西亚) 和减少了有害细菌 (例如,Mucispirillum),积极影响了Firmicutes/Bacteroidetes比率.
结论:
- 奥里桑醇通过减轻肝损伤,炎症和肠道失生症来减轻MASH的显著治疗潜力.
- ORY的机制包括恢复肠道屏障功能,减少内毒性,调节TLR4/NF-κB通路.
- 这些发现支持ORY作为MASH预防和治疗的有希望的药物,强调其在调节肠肝轴上的作用.
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