查加 (Inonotus obliquus) 饮食补充剂的比较研究,使用补充分析技术
Coleton Windsor1, Anna E Kreynes1, Jeff S Chilton1
1North American Reishi, Ltd. D.ba Nammex, Box 1780, Gibsons, BC V0N 1V0, Canada.
International journal of molecular sciences
|April 17, 2025
概括
这项研究开发了分析方法,以区分真正的Chaga (Inonotus obliquus) 和在补充剂中的发酵谷物产品. 这为消费者确保了产品的真实性和质量控制.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 查加 (Inonotus obliquus) 是一种受欢迎的补充剂成分,但由于分析方法不一致,质量控制具有挑战性.
- 有关产品真实性,错误标签和Chaga补充剂的质量存在担忧,特别是那些含有发酵和谷物的补充剂.
- 由于缺乏标准化的方法,因此无法可靠地识别真正的查加与伪造产品之间的差异.
研究的目的:
- 开发和验证一个多分析框架,以区分野生制造的Chaga虫与含有发酵谷物产品的Chaga食补充剂.
- 识别关键的化学标记,以区分真实的Chaga和菌发酵产品.
- 确保功能性和Chaga补充剂行业的消费者安全和市场完整性.
主要方法:
- 采用了高性能薄层染色学 (HPTLC),用各种探测器 (ELSD,PDA/DAD) 的液体染色学 (LC),液体染色学-四极点飞行时间质谱学 (LC-QToF-MS),核磁共振 (NMR) 光谱学,UV-Vis光谱学和-粉分析的组合.
- 分析了包括三类,多糖和黑色素在内的关键标记物.
- 利用NMR指纹和LC-QToF-MS代谢学来进行全面的组成分析.
主要成果:
- 整个查加含有特定的三类 (inotodiol,trametenolic acid) 和类 (osmundacetone),在发酵的谷物中不存在.
- 黑色素吸收度在500nm和缺乏粉 (通过-粉测定和β-葡萄糖量化证实) 有效地区分了查加和发酵谷物产品.
- 核磁共振和LC-QToF-MS分析揭示了野生的Chaga,Inonotus obliquus菌和发酵的谷物产品之间存在显著的组成差异.
结论:
- 综合的多分析方法提供了一个可靠的框架,用于区分真正的Chaga从在食补充剂市场上被误导的产品.
- 这种方法提高了质量控制,确保了产品的真实性,并保护了消费者.
- 这些发现支持对功能性,Chaga和基产品市场的信任和透明度.
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