Tekt3 保护神经巨毛发束的正确功能和形态
Dongmei Su1, Sirun Lu1, Ling Zheng2
1Department of Neuroscience, School of Life Sciences, Shenzhen 518055, China.
International journal of molecular sciences
|April 17, 2025
概括
德-3 (Tekt3) 蛋白稳定斑马鱼毛细胞 (HCs) 中的基诺西利亚,维持感官功能. 神经巨根HC中Tekt3的损失会导致kinosilia缺陷和振动响应受损,突出Tekt3的作用.
科学领域:
- 细胞生物学 细胞生物学
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
背景情况:
- 脊椎动物的听力和平衡依赖于内耳和侧线感觉毛细胞 (HCs).
- HCs利用立体细胞和基因细胞进行机械传导,将机械刺激转化为电信号.
- 德基因 (Tekts) 是微管相关的蛋白质,对纤毛稳定性至关重要,但它们在HC中的定位不清楚.
研究的目的:
- 为了研究斑马鱼毛细胞中Tektin-3 (Tekt3) 的亚细胞定位和功能.
- 确定Tekt3在电影的发展和维护中的作用.
- 探索Tekt3缺乏在感官毛细胞中的功能后果.
主要方法:
- 抗体染色以确定斑马鱼内耳和侧线神经质的Tekt3定位.
- 在野生类型和 tekt3 突变斑马鱼毛细胞中分析 kinocilia 形态.
- 功能性测试包括重要的染料吸收,菌素治疗后的头发细胞再生,以及对振动的震惊反应.
- 在突变斑马鱼中使用野生类型的tekt3 mRNA进行救援实验.
主要成果:
- Tekt3在斑马鱼的内耳 () 和侧线神经毛细胞中表达,主要局部在顶端的细胞质中.
- 斑马鱼的囊和半圆形运河中缺少Tekt3,并且在小鼠耳外毛细胞中显示出类似的分布.
- tekt3突变导致神经巨kinosilia的形态缺陷,导致生命染料摄入受损,再生延迟,振动响应减少.
- 在tekt3突变的功能缺陷被野生类型的tekt3mRNA所挽救.
结论:
- 斑马鱼Tekt3对于维持神经巨基诺西利亚的完整性和功能至关重要,可能会防止低频噪声.
- 细胞内Tekt3分布可能在它的功能中起作用.
- Tekt3可以作为标记物来区分斑马鱼和小鼠的毛细胞亚型,特别是非听觉毛细胞.
更多相关视频
13:04Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings
Published on: May 7, 2016
7.4K
13:58Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
Published on: June 25, 2014
21.3K
相关概念视频
Accessory Structures of the Skin: Hair and Hair Follicles
2.0K
Hair and hair follicles are integral components of the integumentary system. Hair is a filamentous structure composed mainly of a protein called keratin. It is found on the surface of the skin throughout the body, except for areas such as the palms of the hands and soles of the feet.
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
2.0K
Multipotency and Niche of Bulge Stem Cell
3.2K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.2K
Assembly of Complex Microtubule Structures
1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Unrenewable Cells
2.3K
In humans, the photoreceptor cells of the eye and sensory hair cells of the ear lack stem cells. These cells are thus unrenewable and cannot be replaced when they are damaged or destroyed.
Photoreceptors
The retina is composed of several layers and contains specialized cells called photoreceptors. The photoreceptors (rods and cones) change their membrane potential when stimulated by light energy. There are two types of photoreceptors—rods and cones—which differ in the shape of...
Photoreceptors
The retina is composed of several layers and contains specialized cells called photoreceptors. The photoreceptors (rods and cones) change their membrane potential when stimulated by light energy. There are two types of photoreceptors—rods and cones—which differ in the shape of...
2.3K
Hair Cells
39.3K
Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.
39.3K
