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在抑制植物防御和促进病原性方面,Puccinia polysora PpEX 的作用

Qiang Su¹, Xiaofan Qi¹, Kunyu Li¹

¹State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

International journal of molecular sciences

|April 17, 2025

概括

南方玉米生病原体效应剂PpEX通过与ZmMPK3相互作用,抑制玉米中基触发的免疫力. 这种相互作用破坏了MAPK信号通路,帮助病原体的毒性.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
生物化学生化学

背景情况:

由*Puccinia polysora*引起的南方玉米 (SCR) 对玉米生产构成重大威胁.
了解真菌效应物如何操纵宿主免疫力对于开发耐药作物品种至关重要.

研究的目的:

为了阐明Puccinia polysora*效应体PpEX调节玉米免疫反应的机制.
确定PpEX的宿主目标及其在植物防御中的作用.

主要方法:

在感染期间对PpEX表达的定量分析.
评估PpEX表达对植物免疫力和病原体负载的影响.
使用TurboID近距离标记和质谱测量来识别PpEX反应器.
使用分裂-露西法酶和共免疫沉试验来确认蛋白质相互作用.

主要成果:

在感染期间,PpEX被上调,并抑制基诱导的免疫反应,包括MAPK激活和PR基因表达.
表达PpEX的玉米表现出对*Puccinia polysora*的敏感性增加.
ZmMPK3被确定为PpEX的直接相互作用伙伴.
ZmMPK3积极调节玉米对南方玉米生的抵抗力.

结论:

通过与ZmMPK3.3相互作用,PpEX作为一种病毒效应因子.
这种相互作用抑制了MAPK信号通路,从而抑制了植物PTI免疫力.
准PpEX-ZmMPK3相互作用可能是提高玉米对SCR的抗性的一种策略.

关键词:

在PpEX中使用PpEX.普奇尼亚多利索拉在地下在 ZmMPK3 中.效应器效应器是一个有效的效应器.南方的玉米生.

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