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微管完整性与线粒体功能和小鼠植入前胚胎的质量有关

Yu-Ha Shim¹, Min-Jeong Cho², Min-Hee Kang²

¹Department of Biomedical Science, College of Life Science, CHA University, Pocheon 11160, Republic of Korea.

International journal of molecular sciences

|April 17, 2025

概括

微管稳定剂 (MTS),如Taxol和Epothilone D,通过增强线粒体功能,显著改善了小鼠胚胎质量和怀孕结果. 这表明MTS可以帮助在体外培养过程中恢复质量差的胚胎.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
发育生物学是发展生物学.

背景情况:

胚胎质量差是成功辅助生殖医学的一个重要障碍.
线粒体功能障碍与雌性体和雄性体质量降低有关.
卵细胞中的微管完整性与线粒体功能有关.

研究的目的:

研究微管稳定剂 (MTS) 和微管干扰剂 (MTD) 对小鼠植入前胚胎质量和怀孕结果的影响.
评估Taxol,Epothilone D (MTS) 和vinorelbine (MTD) 对线粒体活动和胚胎发育的影响.

主要方法:

使用MTS (Taxol,Epothilone D) 或MTD (vinorelbine) 培养植入前小鼠胚胎.
评估线粒体膜潜力,运动性和胚胎发育率 (胚胎细胞形成,化胚胎细胞).
在将经过治疗的胚胎移植到代孕母亲后,评估怀孕和后代的比例.

主要成果:

MTS治疗显著增加了胚胎中的线粒体膜潜力和运动性.
与MTD治疗和未治疗组相比,在MTS治疗的胚胎中,胚胎形成和胚胎化率更高.
用MTS处理的胚胎移植导致后代数量和分娩率显著增加.

结论:

微管稳定剂 (Taxol,Epothilone D) 增强小鼠植入前胚胎发育能力,与改善的线粒体功能相关.
MTS治疗对胚胎质量和随后的妊娠结果产生积极影响.
在体外培养介质中,MTS具有作为补充剂的潜力,可以提高胚胎的质量和生存能力.

关键词:

细胞骨架细胞骨架交付比率交付比率交付比率交付比率交付比率胚胎胚胎是一个胚胎.微管是微管中的一个.胚胎质量不佳的胚胎质量很差.

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