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肌肉卫星细胞调节的转录基因分析肌肉前脂肪细胞在棕色绵羊中的分化

Xiaochun Xu¹, Cong Zhan¹, Jiaqi Qiao¹

¹Collaborative Innovation Center for Food Production and Safety, School of Biological Science & Engineering, North Minzu University, Yinchuan 750021, China.

International journal of molecular sciences

|April 17, 2025

概括

发现骨肌卫星细胞 (SMSCs) 抑制了羊的肌肉内脂肪沉积. 这种相互作用影响基因表达和脂质代谢,为改善肉质提供了洞察力.

科学领域:

动物科学动物科学
分子生物学分子生物学
肉类科学肉科学

背景情况:

肌肉内脂肪 (IMF) 含量对于肉类质量特征,如柔软性,风味和大理石化,至关重要.
控制IMF沉积的分子机制,特别是细胞与细胞的相互作用,尚未完全理解.

研究的目的:

研究绵羊肌内前脂肪细胞 (IMAdC) 和骨肌卫星细胞 (SMSC) 之间的细胞间相互作用.
阐明SMSCs对IMAdC分化和脂质代谢的调节作用.

主要方法:

使用羊IMAC和SMSC的直接共同培养系统.
在单一培养和共同培养条件下对细胞进行RNA测序和生物信息学分析 (GO,KEGG,联体受体配对).

主要成果:

SMSCs显著抑制了IMAdCs的脂肪生成能力,由改变的基因表达和减少的脂质滴积累证明.
关键的脂肪生成基因 (例如,PPARG) 被下调,而与脂质代谢相关的基因 (例如,ACSL3,IGF1R) 在共同培养的IMAdC中被上调.

结论:

SMSCs在抑制肌肉前脂肪细胞分化和影响脂质代谢方面发挥着重要的调节作用.
这些发现为开发分子策略提供了基础,通过有针对性的干预措施提高绵羊肉质量.

关键词:

棕色绵羊棕色绵羊棕色绵羊细胞通信的细胞通信.肌肉内预先脂细胞.结合体受体对对的对.肌肉卫星细胞肌肉卫星细胞

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