相关实验视频
Updated: May 11, 2025
13:05
Micropatterning and Assembly of 3D Microvessels
Published on: September 9, 2016
11.7K
在芯片上的感应性血管生成:通过内皮层分层进行透光血管桥梁的证据
Sabrina C R Staples1,2, Hao Yin1, Frances S K Sutherland1,3
1Robarts Research Institute, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 3K7, Canada.
概括
吸血感应性血管生成涉及血管分裂,形成穿过光线的内皮细胞桥梁. 研究人员发现,内皮细胞从血管壁分层,形成这些桥梁,这是新血管形成的关键步骤.
科学领域:
- 血管生物学 血管生物学
- 细胞生物学 细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- 输血受体血管生成是产生和重塑微血管网络的关键过程.
- 血管分裂和透光内皮细胞桥梁形成的精确机制仍然不太清楚.
- 了解内皮细胞如何穿过流动的光线对于阐明血管生成至关重要.
研究的目的:
- 为了研究细胞机制基础的透光内皮细胞桥梁形成在输入感受性血管生成期间.
- 在受控的微环境中描述内皮桥的形态和形成过程.
- 为了确定参与内皮细胞光线穿越的分子调节者.
主要方法:
- 利用微血管在芯片系统创建了带有流量敏感内皮细胞的微导体.
- 采用时隔,多平面,三维 (3D) 显微镜实时观察桥梁形成.
- 操纵细胞通路,包括Rho激活和细胞基质相互作用 (非肌肉肌肉蛋白II,α5ß1整体蛋白),以研究桥梁发育.
主要成果:
- 成功生成了微导体,表现出具有多种形态的跨光内皮细胞桥梁,反映了体内发现的.
- 观察到桥梁起源于垂直于流动的内皮细胞,部分脱离血管壁,同时保持它们末端的附着物.
- 证明Rho过度激活阻断了桥梁形成,而削弱细胞基质相互作用则促进了它.
结论:
- 内皮细胞可以通过受控的分层过程积极切断流动的光线.
- 这种新发现的光线进入程序被认为是驱动输入感受性血管生成的基本机制.
- 这些发现为针对病理性血管生成的治疗干预提供了一个新的框架.
相关概念视频
01:10Mechanism of Angiogenesis
5.0K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.0K
01:24Overview of Cell-Matrix Interactions
6.9K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
6.9K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K