受体激酶通路信号通过非转录不连贯的前循环调整
Qian Wang1, Yeon Hee Kang1, Christian S Hardtke1
1Department of Plant Molecular Biology, University of Lausanne, Lausanne CH-1015, Switzerland.
概括
植物受体激酶信号传递涉及膜相关激酶调节器 (MAKR) 蛋白质. 这项研究揭示了MAKR5使用反循环来放大和抑制CLE45信号,调节植物干细胞池.
科学领域:
- 植物分子生物学 植物分子生物学
- 细胞信号传递 细胞信号传递
- 发育生物学是发展生物学.
背景情况:
- 受体激酶信号通路在植物中对于感知配体和调节发育至关重要.
- 内生CLAVATA3/胚胎周围区域 (CLE) 信号量化干细胞池.
- 膜相关激酶调节剂 (MAKR) 蛋白质在植物受体激酶的下游运作.
研究的目的:
- 调查Arabidopsis中MAKR蛋白的功能多样化.
- 为了阐明CLE45信号下游的BARELY ANY MERISTEM 3 (BAM3) 的机制.
- 描述控制MAKR5活动的自我调节反机制.
主要方法:
- 研究了MAKR蛋白的功能相似性.
- 分析了MAKR5对血的招募.
- 研究了MAKR5的产生和降解.
- 研究了5' UTR在MAKR5 mRNA翻译中的作用.
- 评估了MAKR5酸化及其对活性和mRNA水平的影响.
主要成果:
- MAKR3可以模仿MAKR5的功能,表明多样化.
- MAKR5的血招募独立于下游类似受体的细胞质激酶,并取决于膜电荷.
- 通过5'UTR-依赖的mRNA转化,CLE45-BAM3信号放大了MAKR5.
- 由CLE45刺激的MAKR5酸化通过非活化MAKR5并促进其周转而抑制信号传输.
结论:
- MAKR5在一个非转录的不连贯的前循环中起作用.
- 受体激酶信号由联结体触发的翻译放大,并通过联结体触发的蛋白质失活减弱.
- 这种双重调控确保精确控制植物干细胞的增殖和组织的形成.
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