在封闭级别3中使用mopox病毒灭活的协议,以确保动物样本的安全处理
Benedicte Mpia Moni1, Arianna Mahely Hurtado-Monzón2, Jill Van Kessel2
1Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Virology, National Institute for Biomedical Research, Kinshasa, DR Congo; Department of Biology, Faculty of Science and Technology, BP 190 Kinshasa XI, University of Kinshasa, Kinshasa, DR Congo; School of Medicine, Catholic University of Congo, Kinshasa, DR Congo.
STAR protocols
|April 17, 2025
概括
这项研究引入了一种新的协议,用于使用溶解缓冲器在动物样本中使mpox病毒 (MPXV) 不活化. 这种方法可以在较低封闭度的实验室中进行更安全的处理,有助于研究和疫情应对.
科学领域:
- 病毒学 病毒学
- 生物技术是生物技术.
- 公共卫生 公共卫生
背景情况:
- 对于处理传染性mpox病毒 (MPXV) 标本来说,需要高度的生物封闭,这对疫情期间的研究和诊断构成了挑战.
- MPXV的研究和诊断往往受到高生物控制设施严格的安全要求的限制.
研究的目的:
- 开发和验证使用商业可用的溶解缓冲器在受污染的动物样本中禁用MPXV的协议.
- 为了使MPXV样品在较低隔离环境的实验室环境中能够安全处理和下游分析.
主要方法:
- 病毒传播和MPXV的量化.
- 使用商业溶解缓冲器进行MPXV无活化.
- 通过基于细胞的测定和定量聚合酶连锁反应 (qPCR) 验证无活化功效.
主要成果:
- 成功开发和验证了MPXV失活的详细协议.
- 溶解缓冲体方法有效地使受污染的动物样本中的MPXV失活.
- 验证证实治疗后没有传染性MPXV.
结论:
- 本协议提供了一种安全有效的方法,用于在生物封闭水平较低的实验室中管理MPXV污染的样本.
- 这一框架促进了下游的基本应用,如分子测试和研究,从而改善了MPXV爆发管理策略.
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