RNF149通过降低IRF3来调节I型IFN天生的抗病毒免疫反应
Mengyun Wu1, Jiamin Cai1, Guodong Qiao1
1Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China.
PLoS pathogens
|April 17, 2025
概括
RNF149是一种E3泛基因酶,对宿主进行负面调节.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- E3 泛素酶是对抗病毒感染的先天免疫反应的关键调节者.
- 这些酶通过向蛋白质进行降解或激活来控制免疫信号通路.
- 了解参与抗病毒防御的特定E3链酶对于开发治疗策略至关重要.
研究的目的:
- 研究RNF149在宿主对病毒感染的天生的免疫反应中的作用.
- 阐明RNF149影响抗病毒免疫力的分子机制.
主要方法:
- 在对病毒感染的反应中研究了RNF149的表达.
- 评估了RNF149过度表达对IFN-β产生和病毒复制的影响.
- 确定了RNF149与IRF3的相互作用及其无处不在状态 (K27链接和K33链接).
- 通过蛋白质酶路径分析IRF3蛋白质降解.
主要成果:
- 病毒感染上调RNF149的表达.
- 过度表达RNF149导致IFN-β的产生减少,病毒复制增加.
- RNF149与IRF3相互作用,促进其K27和K33相关的泛化和随后的蛋白质体降解.
- 这种机制降低了IRF3蛋白水平,损害了抗病毒反应.
结论:
- RNF149作为宿主对病毒感染的天生的免疫反应的负调节者.
- RNF149针对IRF3进行降解,从而抑制抗病毒信号传输.
- 下调RNF149是一种潜在的治疗策略,可以增强抗病毒免疫力并抑制病毒复制.
更多相关视频
12:43Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
12.0K
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
13.3K
相关概念视频
NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
RNA Interference
25.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.8K
siRNA - Small Interfering RNAs
16.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.3K
Regulation of the Unfolded Protein Response
2.3K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.3K
Immune Response Against Viral Pathogens
472
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
472
