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与艾滋病毒相关的阴茎厌氧会破坏上皮质屏障的完整性

Lane B Buchanan¹, Zhongtian Shao¹, Ronald M Galiwango^2,3

¹Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada.

|April 17, 2025

概括

特定的无氧细菌,称为与血清转化,炎症和免疫细胞 (BASIC) 相关的细菌物种,可能通过破坏阴茎上皮屏障增加HIV-1易感性. 这种干扰补充了它们已知的招募易受细胞的能力.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
泌尿器科泌尿器科泌尿器科泌尿器科

背景情况:

阴茎的微生物组会影响HIV-1的易感性.
与血清转化,炎症和免疫细胞 (BASIC) 相关的细菌物种与增加的HIV-1风险有关.
基本物种对阴茎上皮质屏障完整性的影响尚不清楚.

研究的目的:

调查BASIC物种丰富度与阴茎上皮质屏障完整性之间的关系.
确定BASIC物种是否影响表皮厚度,结蛋白表达和细胞增殖.
探索BASIC物种可能损害上皮屏障的机制.

主要方法:

来自116名接受自愿医疗男性割礼的男性的阴茎组织和抽样的分析.
评估上皮质厚度,克劳丁-1和E-cadherin的表达,以及角质细胞的增殖.
在体外实验中使用重组的人类E-cadherin和前皮上皮细胞来测试特定BASIC物种的降解.

主要成果:

较高的BASIC物种丰度与阴茎组织中Claudin-1和E-cadherin的表达减少相关.
在分泌物中可溶性E-cadherin的升高表明蛋白质分解裂变.
普雷沃特拉·比维亚 (Prevotella bivia) 是一个BASIC物种,在体外直接降解了E-cadherin.
基本物种的丰富性与上皮质厚度增加和 keratinocyte in vivo 的增殖有关.

结论:

基本物种可以通过直接降低阴茎上皮屏障来增强HIV-1易感性.
这种障碍破坏机制是除了招募易受细胞外.
普雷沃特拉生物被确定为参与E-cadherin降解的关键物种.

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