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Mycobacterium canettii和Mycobacterium tuberculosis的比较病变适应:从对菌体酸化,细胞溶液接入和转录组学的测试中获得的见解

Camila do Nascimento Araujo¹, Felipe Silva¹, Cristina Kraemer Zimpel¹

¹Laboratory of Applied Research in Mycobacteria, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Brazil.

Microbes and infection

|April 17, 2025

概括

结核菌菌 (Mtb) 和菌菌 (Mcan) 共有诸如菌体操纵之类的祖先特征. 遗传变异,特别是在双组件系统中,驱动基因表达和向宿主适应的进化差异.

科学领域:

微生物学微生物学
进化生物学进化生物学
遗传学是一个遗传学.

背景情况:

结核菌 (Mtb) 和它的祖先Mycobacterium canettii (Mcan) 之间的遗传和分子区别尚未得到充分理解.
研究这些差异可以揭示Mtb病原性的进化路径.

研究的目的:

为了比较Mtb和Mcan如何调节胞体酸化,并在巨细胞内获得细胞质的访问.
为了分析Mtb和Mcan的体外转录组.
识别导致表型分歧的遗传和分子因素.

主要方法:

用光谱光度测量来监测含有巨细胞的真空孔中的pH值变化.
一个单细胞的Förster共振能量转移 (FRET) 协议评估了菌根细菌的细胞质接入.
进行了转录和遗传分析,以比较基因表达和识别突变.

主要成果:

Mtb和Mcan都抑制了细胞酸性化,并进入了巨细胞溶.
两个组成系统 (PhoPR,SenX3-RegX3,DevRS/DosRS) 和乳酸脱酶和espACD等基因的突变影响基因表达的差异.
与Mtb相比,Mcan表现出高调铁和二代谢基因,这表明金属在进化中的作用.
Mtb显示Rho终结因子的表达增加,表明基因表达调节的潜在差异.

结论:

发酵体调节和细胞质接入是古老的特征,并不仅限于Mtb的致病性生活方式.
受遗传变异影响的基因表达调节,在从环境中的Mcan类祖先到宿主适应的Mtb的进化中发挥了关键作用.

关键词:

细胞层可以进入细胞层.基因调节基因调节铁的收购铁的收购这种细菌是Mycobacterium canettii.结核病菌菌菌的结核病菌的结核病菌.基体酸化发生在基体中.

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