通过与铁过载相关的氧化应激引起的视网膜损伤中的蓝光诱导铁
Yutong Xia1, Qianjie Yang1, Liyue Zhang1
1Department of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
Journal of environmental sciences (China)
|April 17, 2025
概括
蓝光暴露会通过铁亡引起视网膜损伤,其特征是铁过载和氧化应激. 德费洛克萨甲基酸盐治疗有效地保护了这种损伤,为光引起的眼睛疾病提供了潜在的治疗策略.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 电子设备造成的光污染是一个越来越令人担忧的问题.
- 众所周知,蓝光 (BL) 可能会损害视网膜.
- 铁亡,一种编程细胞死亡,与视网膜疾病有关,但其与BL损伤的联系尚不清楚.
研究的目的:
- 为了研究铁死在蓝光诱导的视网膜损伤中的作用.
- 阐明在视网膜中BL诱导的铁亡的潜在机制.
主要方法:
- 利用小鼠模型和培养的ARPE-19细胞暴露在蓝光下.
- 使用H&E染色, fundus摄影,免疫染色和TEM评估视网膜结构和形态.
- 通过DCFH-DA,C11-BODIPY和FeRhoNoxTM-1探针在体外测量铁化水平.
- 通过实时PCR和西式涂抹分析了基因和蛋白质表达.
主要成果:
- 蓝光暴露导致视网膜细胞的铁过载和氧化应激.
- 观察到与铁死相关的标记物 (TFR1,ACSL4,HO-1) 的增加和标记物 (SOD2,CAT,GPX4) 的减少.
- 铁化剂德费罗胺酸盐显著降低了脂质过氧化,并在体内和体外减轻了视网膜损伤.
结论:
- 铁亡是蓝光诱导的视网膜损伤的一个关键机制.
- 铁过载和氧化应激是这个过程的关键组成部分.
- 德费罗胺酸显示出缓解蓝光诱导的视网膜损伤的治疗潜力.
更多相关视频
10:39Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
Published on: April 14, 2023
2.3K
10:00Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020
2.5K
相关概念视频
01:16Necrosis
3.6K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
3.6K
01:22Photoreceptors and Visual Pathways
5.5K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
5.5K