Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

3D pathology-guided microdissection.

Nature methods·2026
Same author

Assessing the Effects of a 3-Dimensional (3D) Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

Associations of the CHG index combined with obesity indicators with cardiovascular disease in early CKM syndrome using CHARLS data.

Scientific reports·2026
Same author

A systematic comparison of cavitation regimes and histotripsy efficiency across pulse duration and repetition rate in a fibrous tissue mimicking phantom.

Research square·2026
Same author

De Novo Design of Near-Infrared Fluorescence-Activating Proteins.

Journal of the American Chemical Society·2026
Same author

Interpretable machine learning of non-traditional lipid indices for diagnostic classification of CHD in patients with comorbid MASLD and T2DM: a multicenter study.

Frontiers in nutrition·2026

相关实验视频

Updated: May 11, 2026

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

18.2K

用光学显微镜对3D细胞培养进行成像.

Huai-Ching Hsieh1,2, Qinghua Han1,2, David Brenes2

  • 1Department of Bioengineering, University of Washington, Seattle, WA, USA.

Nature methods
|April 17, 2025
PubMed
概括

本综述探讨了三维 (3D) 细胞培养的显微镜技术,并比较了2D和3D成像方法. 它向用户指导最佳的成像策略,并突出了未来高分辨率显微镜创新的领域.

更多相关视频

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.1K
Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
07:35

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

11.7K

相关实验视频

Last Updated: May 11, 2026

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

18.2K
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.1K
Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
07:35

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

11.7K

科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 三维 (3D) 细胞培养越来越多地模拟复杂的组织,超过传统的二维 (2D) 培养.
  • 先进的显微镜对于分析这些复杂的3D模型至关重要.

研究的目的:

  • 审查用于3D细胞培养监测的光学显微镜应用.
  • 为了比较各种显微镜技术的优缺点.
  • 确定在3D细胞培养成像中创新的需求和机会.

主要方法:

  • 总结了3D细胞培养中使用的流行的光学显微镜方法.
  • 讨论了显微镜技术对不同类型的调查的适用性.
  • 分析了2D与3D成像方法的优缺点.

主要成果:

  • 确定了适用于3D细胞培养的关键显微镜技术.
  • 概述了每个方法的具体好处和局限性.
  • 需要技术进步以改善成像的突出领域.

结论:

  • 显微镜的选择取决于3D细胞培养的具体研究目标.
  • 需要在高分辨率成像方面的创新才能充分利用3D文化潜力.
  • 为研究人员和工程师选择最佳成像方法提供了指导.