皮下母体复合体通过防止SPIN1的核进入来保护小鼠卵细胞到胚胎的过渡
Chengpeng Xu1,2, Dandan Qin1, Xukun Lu3
1State Key Laboratory of Organ Regeneration and Reconstruction, Beijing Institute for Stem Cell and Regenerative Medicine, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Nature structural & molecular biology
|April 17, 2025
概括
研究人员在皮层下母体复合体 (SCMC) 中发现了一种基因素读取器SPIN1. 菲利亚蛋白控制着SPIN1的作用.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 控制哺乳动物卵细胞和早期胚胎中核事件的细胞质调节剂尚不清楚.
- 皮下母体复合体 (SCMC) 是一个细胞质复合体,参与核事件.
- 目前尚不清楚SCMC影响核过程的确切机制.
研究的目的:
- 确定SCMC的新型组件,并阐明它们在核监管中的作用.
- 研究SPIN1在SCMC中的功能及其对早期胚胎发育的影响.
- 了解FILIA和SPIN1.1之间的监管关系.
主要方法:
- 同免疫沉以评估蛋白质相互作用.
- 西方涂抹用于评估蛋白质表达水平.
- 免疫光显微镜以确定蛋白质定位.
- 对H3K4me3重编程,胚胎基因组激活和突变胚胎胚胎发育的分析.
主要成果:
- 作为SCMC的新成员,SPIN1是一款基因素甲基化读取器.
- 菲利亚与SPIN1直接相互作用,调节其表达和细胞质局部化.
- 减少FILIA导致SPIN1表达的减少及其异常的核转位.
- 核SPIN1破坏了H3K4me3重编程,胚胎基因组激活和胚胎发育.
- 抑制SPIN1-H3K4me3相互作用部分挽救发育缺陷.
- 在H3K4me3结合方面,SPIN1与KDM5B竞争,影响脱甲基化.
结论:
- 为了保持SCMC的完整性和功能,FILIA对SPIN1的调节至关重要.
- 异常的SPIN1核定位破坏了重要的表观遗传重编程事件.
- SPIN1与H3K4me3的相互作用在调节组织蛋白脱甲基化中起作用.
- 这项研究揭示了SCMC中一种新的调节机制,这对于哺乳动物卵细胞转化为胚胎至关重要.
相关概念视频
01:57Meiosis II
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
02:07Oogenesis
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
03:17Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
02:19The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
02:02Meiosis II
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
01:22Oogenesis
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...