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在前列腺癌患者中,循环的先天性淋巴细胞失调
Daniela Claudia Maresca1, Evelina La Civita2, Benedetta Romano1
1Department of Pharmacy, School of Medicine, University of Naples Federico II, 80138, Naples, Italy.
Cellular & molecular biology letters
|April 17, 2025
概括
前列腺癌 (PCa) 显示前瘤先天性淋巴细胞 (ILC2s) 的增加和抗瘤ILC1s的减少. 这些ILC子集是PCa治疗的潜在治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
背景情况:
- 前列腺癌 (PCa) 是一个重要的全球健康问题,特别是在50岁以上的男性中.
- 尽管PCa预后和治疗方面取得了进展,但仍需要新的治疗点.
- 天生的淋巴细胞 (ILC) 调节瘤免疫力,但它们在PCa中的作用尚不清楚.
研究的目的:
- 在PCa患者中调查ILC子集的表型和功能概况.
- 确定ILCs对PCa免疫和进展的贡献.
- 根据格里森得分和前列腺特异性抗原 (PSA) 水平分层ILC资料.
主要方法:
- 从PCa患者和健康捐赠者中分离的外周血液单核细胞 (PBMCs).
- 使用流式细胞计量评估ILC频率和活性.
- 分析了血清细胞因子水平,并与PCa细胞系进行了体外共同培养实验.
主要成果:
- 在PCa患者中观察到增加的ILC2频率和减少的ILC1频率.
- 较高的ILC2频率与较高的PSA值相关.
- 过度活化的ILC2s产生IL-13和IL-5;ILC1s显示TNF-α和IFN-γ分泌减少.
- 在PCa患者中发现ILC2-激活细胞因子 (IL-33,IL-18,PGD2) 的血清水平升高.
- PCa细胞系增强了ILC2的活动,而来自ILC2s的IL-13促进了PCa细胞的迁移和入侵.
结论:
- PCa的特点是失调的ILC形状,ILC2占主导地位,活动增加.
- 降低ILC1功能和增加ILC2活动有助于PCa的进展.
- ILC1和ILC2都代表了PCa治疗的潜在治疗点.
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