海鲜产品中黄金葡萄球菌的流行率,毒性因素和抗生素耐药性
Rasool Esmaili Derke1, Ebrahim Rahimi2, Amir Shakerian1
1Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
BMC infectious diseases
|April 17, 2025
概括
黄金葡萄球菌 (S. aureus) 在海鲜中很常见,许多菌株表现出抗生素耐药性和毒性因素. 良好的卫生习惯对于预防受污染的海鲜引起的食物传播疾病至关重要.
科学领域:
- 食品微生物学 食品微生物学
- 公共卫生 公共卫生
- 细菌病原体的产生
背景情况:
- 海鲜的细菌污染对食源性疾病构成重大风险.
- 黄金葡萄球菌 (S. aureus) 是一种关键的病原体,在海产品安全方面令人担忧.
研究的目的:
- 为了确定S. aureus在各种海鲜产品中的流行率.
- 鉴定S. aureus菌株的毒性因子和抗生素耐药性概况.
- 评估甲素耐药黄金色杆菌 (MRSA) 和相关耐药基因的存在.
主要方法:
- 从伊朗的沙赫雷科德收集了460个海鲜样本 (鱼,,,龙,).
- 通过ISO标准和PCR (16S rRNA,核基因) 来分离和鉴定金黄色细菌.
- 通过PCR评估了抗生素敏感性 (Kirby-Bauer),并通过PCR检测了毒性 (肠毒素) 和耐药基因 (mecA, blaZ).
主要成果:
- 在所有海鲜类型的样本中,S. aureus 在27.83%的样本中检测到.
- 在大多数样本中发现了MRSA,不包括和.
- 发现病毒性基因 (Sea,Seb,Sed,Sec,See) 的流行以及对青素G和安培素的高耐药性.
- 在超过50%的分离物中发现了mecA基因; blaZ是最常见的耐药基因. 利尼佐利德显示100%的敏感性.
结论:
- 与海鲜相关的金黄色菌经常含有毒性因素和抗生素耐药性基因.
- 这些发现强调了在海鲜加工中需要严格的良好卫生实践 (GHP).
- 警的食品安全措施对于控制通过海鲜传染的金黄色细菌至关重要.
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