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注射,浸泡和口服输送系统诱导RNA干扰介导的沉默在虫,Ferrisia gilli Gullan (Hemiptera: Pseudococcidae) 中

Raman Bansal¹, Esther Fernandez¹

¹USDA-ARS, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, USA.

Pest management science

|April 18, 2025

概括

RNA干扰 (RNAi) 有效地控制了花生粉虫,Ferrisia gilli. 通过局部养口服输送显示出对现场应用的希望,减少了的生存率和基因表达.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
虫害管理虫害管理虫害管理

背景情况:

费里西亚米虫 (Ferrisia gilli mealybug) 是加利福尼亚州的一种重要 pistachio 害虫.
有限的化学控制选择需要新的害虫管理策略.
RNA干扰 (RNAi) 为F.gilli控制提供了一种新的作用方式.

研究的目的:

为了研究费里西亚的RNAi功能.
评估用于害虫控制的不同RNA输送方法.
为了评估向αCOP基因的有效性.

主要方法:

在F.gilli中探索RNAi,使用注射,浸泡和口服.
针对重要的真核生物基因αCOP.
优化局部养,用于精灵和成人的口服分娩.

主要成果:

所有的输送方法都诱导了RNAi,注射显示了76%的转录减少.
通过局部食进行口服,实现了高达65%的转录减少.
局部食显著降低了的生存率,但并没有降低成人的生存率.

结论:

RNAi是一种可行的Ferrisia gilli的控制方法.
口服,特别是局部食,支持基于现场的RNAi控制策略.
局部养方法适用于大规模的基因发现和现场评估.

关键词:

作为一个共聚物,它是coatomer.送货送货送货送货送货送货表达方式表达方式表达方式表达梅利布格 (mealybugbug) 是一个有趣的虫.沉默器是一种沉默器.

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