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在B.B.中对控制花和植物结构的BnaSBT基因进行映射和分子标记的开发. 纳普斯 (napus) 是一个

Meng Jiang¹, Jingming Li¹, Yingying Huang¹

¹National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Centre of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070 China.

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概括

研究人员确定了一个主导基因,BnaSBT,控制了Brassica napus中的分散芽特征,影响了植物结构. 这一发现有助于通过提供链接的分子标记物来培育用于机械收获的菜品种.

科学领域:

植物遗传学和育种.
植物架构的分子机制.

背景情况:

对于中国种植者来说,开发具有机械收获理想植物架构的新番茄 (Brassica napus) 品种至关重要.
在B. napus中控制植物结构的基因很少被确定,这阻碍了繁殖的进展.

研究的目的:

描述一个矮而紧的Brassica napus线 (SX3) 具有分散芽的特征.
为了确定负责分散芽特征 (SBT) 的基因及其对植物高度和树枝角度的类效应.

主要方法:

对隔离种群的基因分析来自交叉SX3与高高的,松散的建筑线.
大量分离分析测序 (BSA-seq) 来映射负责任的基因.
RNA测序 (RNA-seq) 用于分析对比的表型之间的基因表达差异.
进行比较测序以识别候选基因.

主要成果:

分散芽特征 (SBT) 是由单个主导基因BnaSBT在染色体A10上控制的.
似乎BnaSBT是一种影响植物高度和树枝角的类基因.
BSA-seq将BnaSBT映射到ChrA10.0上的4.15Mb区域.
RNA-seq揭示了参与氨基酸/脂质代谢和MADS盒转录因子的基因的差异表达.
对于BnaSBT的候选基因包括BnaA10.SEP1,BnaA10.AGL15,BnaA10.GLN1-4和BnaA10.AGP15. 这两种基因.

结论:

BnaSBT是一种关键的基因,控制了Brassica napus中的植物结构.
与BnaSBT相关的分子标记物被开发用于标记物辅助选择.
这些发现为培育理想植物类型和理解B. napus.花朵发育提供了宝贵的生殖质和理论基础.

关键词:

布拉斯卡纳普斯 (Brassica napus) 是一种植物.矮和紧的植物架构矮和紧的植物架构基因映射基因映射基因映射花朵和植物架构的花朵和植物架构.在RNA-seqqq.散落的芽散落的芽.

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