Circ_0098181与PKM2结合,可以减轻肝纤维化
Yuan-Yuan Luo1, Ya-Ping Guan1, Hong-Fei Zhan1
1Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Frontiers in pharmacology
|April 18, 2025
概括
循环RNAcirc_0098181在肝纤维化中减少. 恢复circ_0098181通过向PKM2来抑制肝星细胞激活和肝纤维化,为肝硬化提供治疗潜力.
科学领域:
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
- 在RNA生物学,RNA生物学.
背景情况:
- 肝硬化是一种严重的健康问题,纤维化是其关键的病理过程.
- 循环RNAs (circRNAs) 与肝纤维化有关,但关键参与者和机制在很大程度上仍未知.
- 这项研究研究了circ_0098181在肝纤维化中的作用和机制.
研究的目的:
- 为了确定circ_0098181对肝脏纤维生成的影响.
- 为了阐明circ_0098181在肝纤维化中的潜在分子机制.
- 探索circ_0098181作为肝硬化治疗的潜在治疗标.
主要方法:
- RNA测序确定了人体肝硬化组织中的circRNA配置文件.
- 试验室 (HSC) 和体内 (CCl4诱导) 模型被用于评估circ_0098181的功能.
- 通过RNA下拉,质谱学,RIP和qRT-PCR阐明了机制.
主要成果:
- Circ_0098181的度在纤维化的肝组织和激活的肝星细胞 (HSC) 中下降.
- 外源性circ_0098181的使用抑制了HSC的激活,增殖,迁移,并改善了肝纤维化.
- Circ_0098181向酸盐激酶M2 (PKM2),抑制其核转位和酸化,并抑制促炎性细胞因子.
结论:
- Circ_0098181在肝硬化中表现出显著的抗纤维效应.
- 该机制涉及向PKM2并抑制肝炎炎症.
- Circ_0098181作为肝硬化治疗剂具有前途.
关键词:
在RNA1 (ADAR1) 上作用的腺氨酸脱氨酶.循环RNAs (循环RNAs) 是一种循环RNA.肝脏纤维化 肝脏纤维化有助于炎症的细胞因子.酸盐激酶M2的 pyruvate 激酶 M2 的 pyruvate 激酶 M2 的 pyruvate 激酶更多相关视频
15:43Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
18.1K
10:42Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
Published on: May 13, 2016
9.1K
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
3.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.3K
01:24Liver Regeneration
3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K
01:58Cell Specific Gene Expression
13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K