通过代谢和酶工程,在Yarrowia lipolytica中高效地生产阿斯塔桑
Chalak Najat Abdullah1,2,3, Mengsu Liu1,3, Qihang Chen1,3
1School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.
Synthetic and systems biotechnology
|April 18, 2025
概括
研究人员对Yarrowia lipolytica进行了高水平的阿斯塔山丁生产的工程,通过代谢工程和发酵实现了2820 mg/L. 这表明这种有价值的抗氧化剂的229倍改善.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物生产 微生物生产
背景情况:
- 作为一种强有力的抗氧化类胡卜素抗氧化剂,阿斯坦丁因其复杂的途径而面临着新生生物合成的挑战.
- 雅罗维亚 (Yarrowia lipolytica) 是一个有希望的宿主,用于微生物生产有价值的化合物.
- 对于制药和营养食品应用而言,高效和具有成本效益的阿斯塔山丁生产至关重要.
研究的目的:
- 使用成本效益高的方法,对Yarrowia lipolytica进行高水平的阿斯塔山丁生产的工程设计.
- 为了克服阿斯塔克桑复杂的生物合成途径的挑战.
- 为了实现阿斯塔山丁的工业相关产量和生产率.
主要方法:
- 在Yarrowia lipolytica中构建了一个β-胡卜素平台菌株.
- 集成和增强的关键酶 (藻类β-胡卜素基酶和基酶) 用于阿斯塔山丁合成.
- 采用代基因整合,子器官细胞路径向和辅性标记物恢复.
- 优化了介质成分,并使用了料批发发酵策略.
主要成果:
- 在摇瓶平台菌株中达到411.7mg/L的β-胡卜素.
- 在初始酶集成和复合体形成后,产生了12.3 mg/L和41.0 mg/L的阿斯塔桑丁.
- 通过代基因组集成和媒介优化,增加了阿斯塔山丁的产量,达到172.1 mg/L和237.3 mg/L.
- 通过食批发发酵达到2820 mg/L的最终阿斯塔山丁度,提高了229倍.
结论:
- 亚罗维亚脂质菌的代谢工程对于促进阿斯塔克桑的生产是非常有效的.
- 开发的策略显著提高了阿斯塔克桑产量和生产力.
- 这项工作为Yarrowia lipolytica的阿斯塔桑生产树立了新的基准,为工业应用铺平了道路.
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